Gupta Vivek Kumar, Waugh Courtney Alice, Ziklo Noa, Huston Wilhelmina M, Hocking Jane S, Timms Peter
Department of Microbiology, Faculty of Science, Education, Health and Engineering, University of Sunshine Coast, Brisbane, Australia; Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India.
Department of Microbiology, Faculty of Science, Education, Health and Engineering, University of Sunshine Coast, Brisbane, Australia.
Indian J Med Microbiol. 2017 Jul-Sep;35(3):394-401. doi: 10.4103/ijmm.IJMM_17_1.
Chlamydia trachomatis is the etiological agent for the most prevalent bacterial sexually transmitted infection in both developed and developing countries. The aim of present study was to characterize the antibody response between two groups of individuals, having either a single C. trachomatis infection and or repeated infections.
Current study consisted of two groups, one with an initial Chlamydia infection and a second with repeated infections. A titre based estimation of specific serum (IgG and IgA) levels using ELISA were performed, which further validated by western blot. In vitro neutralizing ability of each patient's serum against both homologous and heterologous strains was also determined.
Individuals infected with one of the C. trachomatis serovars D, E or K exhibited a strong systemic antibody response as characterized by ELISA and western blot. These individuals may have developed at least some level of protection as they only represented single infection. By comparison, individuals infected with serovar D, E or F that exhibited low systemic antibody response often presented repeated C. trachomatis infections, suggesting an association with poor immune response. An in vitro neutralizing level of 60-90% was observed in the human sera against homologous serovar D and two heterologous C. trachomatis serovars E and K, compared to <40% against heterologous serovars F.
Individuals infected with serovars D and K showed a potential association between circulating antibody response and re-infection risk. While the patients infected with serovars E showed a disconnection between systemic antibody response and re-infection risk.
沙眼衣原体是发达国家和发展中国家最常见的细菌性性传播感染的病原体。本研究的目的是描述两组个体之间的抗体反应特征，这两组个体分别患有单一沙眼衣原体感染或反复感染。
本研究包括两组，一组为初次感染沙眼衣原体，另一组为反复感染。使用酶联免疫吸附测定法（ELISA）对特异性血清（IgG和IgA）水平进行基于滴度的估计，并通过蛋白质印迹法进一步验证。还测定了每位患者血清对同源和异源菌株的体外中和能力。
感染沙眼衣原体血清型D、E或K之一的个体表现出强烈的全身抗体反应，这通过ELISA和蛋白质印迹法得以表征。这些个体可能至少产生了一定程度的保护，因为他们仅表现为单一感染。相比之下，感染血清型D、E或F且全身抗体反应较低的个体经常出现沙眼衣原体反复感染，这表明与免疫反应不佳有关。在人血清中观察到针对同源血清型D以及两种异源沙眼衣原体血清型E和K的体外中和水平为60 - 90%，而针对异源血清型F的中和水平则小于40%。
感染血清型D和K的个体显示出循环抗体反应与再感染风险之间存在潜在关联。而感染血清型E的患者则显示出全身抗体反应与再感染风险之间没有关联。
