Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, 605014, India.
Probiotics Antimicrob Proteins. 2018 Dec;10(4):775-786. doi: 10.1007/s12602-017-9341-3.
Lactobacillus mucosae strain AN1 isolated from sheep milk and characterized for its probiotic suitability. In vitro evaluation of critical gut endurance properties of this strain were assessed by different screening methods such as bile salt, gastric acid, lysozyme tolerance assays, hemolytic, cholesterol reduction properties, and HT-29 cell line adhesion assay. Antibacterial peptide from this strain was purified using ammonium sulphate precipitation, gel filtration chromatography and reverse-phase HPLC. The molecular mass of peptides was determined by Tricine-SDS-PAGE and confirmed by matrix-assisted laser desorption ionization-time of flight mass spectroscopy (MALDI-TOF-MS). Purified peptide was named as AN1 having a molecular mass of 10.66 kDa. Helical structures of peptide were determined using circular dichroism spectroscopy. Stability of peptide AN1 towards different parameters such as pH, temperature, organic solvents, proteolytic, and glycolytic enzymes was also analyzed.
从绵羊奶中分离出的黏膜乳杆菌 AN1 菌株及其益生菌适用性的特征。通过不同的筛选方法，如胆盐、胃酸、溶菌酶耐受性试验、溶血、胆固醇降低特性和 HT-29 细胞系黏附试验，评估了该菌株的关键肠道耐力特性。使用硫酸铵沉淀、凝胶过滤层析和反相 HPLC 从该菌株中纯化抗菌肽。肽的分子量通过 Tricine-SDS-PAGE 确定，并通过基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF-MS) 确认。纯化的肽被命名为 AN1，分子量为 10.66 kDa。使用圆二色性光谱确定肽的螺旋结构。还分析了肽 AN1 对不同参数（如 pH 值、温度、有机溶剂、蛋白水解和糖酵解酶）的稳定性。
