Li Yanpeng, Ma Hui, Gan Lin, Gong Andong, Zhang Haibin, Liu Deming, Sun Qizhen
School of Optical and Electronic Information, Huazhong University of Science and Technology Wuhan, Hubei, China.
College of Life Science, Xinyang Normal University Xinyang, Henan, China.
J Biophotonics. 2017 Dec 7:e201700162. doi: 10.1002/jbio.201700162.
Escherichia coli (E. coli) bacteria have been found to be responsible for various health outbreaks caused by contaminated food and water. Accurate and rapid test of E. coli is thus crucial for protecting the public health. A fast-response, label-free bacteriophage-based detection of E. coli using multimode microfiber probe is proposed and demonstrated in this paper. Due to the abrupt taper and subwavelength diameter, different modes are excited and guided in the microfiber as evanescent field that can interact with surrounding E. coli directly. The change of E. coli concentration and corresponding binding of E. coli bacteria on microfiber surface will lead to the shift of optical spectrum, which can be exploited for the application of biosensing. The proposed method is capable of reliable detection of E. coli concentration as low as 10 cfu/mL within the range of 10 to 10 cfu/mL. Owing to the advantages of high sensitivity and fast response, the microfiber probe has great potential application in the fields of environment monitoring and food safety.
已发现大肠杆菌会导致由受污染的食物和水引发的各种健康问题。因此，准确、快速地检测大肠杆菌对于保护公众健康至关重要。本文提出并展示了一种基于多模微纤维探针的快速响应、无标记的大肠杆菌噬菌体检测方法。由于微纤维的突然变细和亚波长直径，不同模式在微纤维中被激发并以倏逝场的形式传播，该倏逝场可直接与周围的大肠杆菌相互作用。大肠杆菌浓度的变化以及大肠杆菌在微纤维表面的相应结合会导致光谱的移动，这可用于生物传感应用。所提出的方法能够在10至10⁵ cfu/mL的范围内可靠地检测低至10 cfu/mL的大肠杆菌浓度。由于具有高灵敏度和快速响应的优点，微纤维探针在环境监测和食品安全领域具有巨大的潜在应用价值。
