Invasive Insect Biocontrol and Behavior Laboratory, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD 20705, USA.
Electron and Confocal Microscopy Unit, Beltsville Agricultural Research Center, USDA Agricultural Research Service, Beltsville, MD 20705, USA.
Viruses. 2017 Oct 21;9(10):307. doi: 10.3390/v9100307.
Operophtera brumata nucleopolyhedrovirus (OpbuNPV) infects the larvae of the winter moth, . As part of an effort to explore the pesticidal potential of OpbuNPV, an isolate of this virus from Massachusetts (USA)-OpbuNPV-MA-was characterized by electron microscopy of OpbuNPV occlusion bodies (OBs) and by sequencing of the viral genome. The OBs of OpbuNPV-MA consisted of irregular polyhedra and contained virions consisting of a single rod-shaped nucleocapsid within each envelope. Presumptive cypovirus OBs were also detected in sections of the OB preparation. The OpbuNPV-MA genome assembly yielded a circular contig of 119,054 bp and was found to contain little genetic variation, with most polymorphisms occurring at a frequency of < 6%. A total of 130 open reading frames (ORFs) were annotated, including the 38 core genes of , along with five homologous repeat () regions. The results of BLASTp and phylogenetic analysis with selected ORFs indicated that OpbuNPV-MA is not closely related to other alphabaculoviruses. Phylogenies based on concatenated core gene amino acid sequence alignments placed OpbuNPV-MA on a basal branch lying outside other alphabaculovirus clades. These results indicate that OpbuNPV-MA represents a divergent baculovirus lineage that appeared early during the diversification of genus .
舞毒蛾核多角体病毒（OpbuNPV）感染舞毒蛾的幼虫。作为探索 OpbuNPV 杀虫潜力的一部分，对来自美国马萨诸塞州的该病毒分离株（OpbuNPV-MA）进行了特征描述，方法是通过电子显微镜观察 OpbuNPV 包埋体（OB）和病毒基因组测序。OpbuNPV-MA 的 OB 由不规则多面体组成，每个包膜内包含一个由单个杆状核衣壳组成的病毒粒子。在 OB 制剂的切片中还检测到了推定的 cypovirus OB。OpbuNPV-MA 基因组组装得到了一个 119,054 bp 的环状连续体，发现其遗传变异很小，大多数多态性发生的频率<6%。共注释了 130 个开放阅读框（ORF），包括 38 个核心基因和 5 个同源重复（）区域。BLASTp 和与选定 ORF 的系统发育分析的结果表明，OpbuNPV-MA 与其他α杆状病毒关系不密切。基于串联核心基因氨基酸序列比对的系统发育树将 OpbuNPV-MA 置于其他α杆状病毒分支之外的一个基础分支上。这些结果表明，OpbuNPV-MA 代表了一个分化的杆状病毒谱系，它在属的多样化早期就出现了。
