Laboratory of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, 80-307, Gdansk, Poland.
Biological Threats Identification and Countermeasure Center, General Karol Kaczkowski Military Institute of Hygiene and Epidemiology, 24-100, Pulawy, Poland.
Virus Genes. 2024 Jun;60(3):275-286. doi: 10.1007/s11262-024-02062-x. Epub 2024 Apr 9.
LesaNPV (Leucoma salicis nucleopolyhedrovirus) is an alphabaculovirus group Ib. Potentially, it can be an eco-friendly agent to control the white satin moth Leucoma salicis population. In this study, we have established the relationship between LesaNPV and other closely related alphabaculoviruses. Environmental samples of late instar of white satin moth collected in Poland infected with baculovirus have been homogenized, polyhedra were purified and subjected to scanning and transmission electron microscopy. Viral DNA was sequenced using the Illumina platform and the whole-genome sequence was established by de novo assembly of paired reads. Genome annotation and phylogenetic analyses were performed with the use of bioinformatics tools. The genome of LesaNPV is 132 549 bp long with 154 ORFs and 54.9% GC content. Whole-genome sequencing revealed deletion of dUTPase as well as ribonucleoside reductases small and large subunits region in LesaNPV genome compared to Dasychira pudibunda nucleopolyhedrovirus (DapuNPV) and Orgyia pseudotsugata multiple nucleopolyhedrovirus (OpMNPV) where this region is complete. Phylogenetic analysis of Baculoviridae family members showed that LesaNPV is less divergent from a common ancestor than closely related species DapuNPV and OpMNPV. This is interesting because their hosts do not occur in the same area. The baculoviruses described in this manuscript are probably isolates of one species and could be assigned to recently denominated species Alphabaculovirus orpseudotsugatae, historically originating from OpMNPV. This finding could have significant implications for the classification and understanding of the phylogeographical spread of baculoviruses.
LesaNPV（柳蓝叶甲核型多角体病毒）是一种α-杆状病毒组 Ib。它有可能成为一种环保型制剂，用以控制白纹毒蛾 Leucoma salicis 的种群。在本研究中，我们建立了 LesaNPV 与其他密切相关的α-杆状病毒之间的关系。在波兰，收集的白纹毒蛾后期幼虫的环境样本感染了杆状病毒，对这些样本进行匀浆，纯化多角体，并用扫描和透射电子显微镜进行观察。使用 Illumina 平台对病毒 DNA 进行测序，并通过配对读取的从头组装建立全基因组序列。使用生物信息学工具进行基因组注释和系统发育分析。LesaNPV 的基因组长 132549bp，包含 154 个 ORF 和 54.9%的 GC 含量。全基因组测序显示，与 Dasychira pudibunda 核型多角体病毒（DapuNPV）和 Orgyia pseudotsugata 多角体病毒（OpMNPV）相比，LesaNPV 基因组中缺失了 dUTPase 以及核糖核苷酸还原酶小亚基和大亚基区域，而这一区域在 DapuNPV 和 OpMNPV 中是完整的。杆状病毒科家族成员的系统发育分析表明，与亲缘关系较近的 DapuNPV 和 OpMNPV 相比，LesaNPV 与其共同祖先的分歧程度较小。这很有趣，因为它们的宿主并不出现在同一地区。本文中描述的杆状病毒可能是一个物种的分离株，并且可以被分配到最近命名的物种 Alphabaculovirus orpseudotsugatae，该物种历史上起源于 OpMNPV。这一发现可能对杆状病毒的分类和理解其系统地理学传播具有重要意义。
