Key Laboratory of Plant Resource Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
School of Life Sciences, University of Chinese Academy of Sciences, Beijing 100039, China.
Toxins (Basel). 2017 Oct 21;9(10):335. doi: 10.3390/toxins9100335.
Most tropical fruits after harvest are very perishable because of fungal infection. Since some pathogenic fungi can produce hazardous compounds such as mycotoxins, novel rapid and effective methods to assess those hazardous compounds are urgently needed. Herein we report that sp. Q67, a luminescent bacterium, can be used to rapidly assess the toxicities of mycotoxins and cultures from mycotoxin-producing pathogens. A good correlation (² > 0.98) between concentrations of the mycotoxins (fumonisin B1, deoxynivalenol, zearalenone, ochratoxin A, patulin, and citrinin) and the luminous intensity of sp. Q67 was obtained. Furthermore, significant correlations (² > 0.96) between the amount of mycotoxin and the luminous intensity from the cultures of 10 major mycotoxin-producing pathogens were also observed. In addition, (half-maximal inhibitory concentration (IC) = 17.49%) exhibited greater luminescence suppression than (IC = 92.56%) or (IC = 28.61%), which was in agreement with the existing higher levels of fumonisin B1, fumonisin B2, and deoxynivalenol, which were measured by high-performance liquid chromatography-tandem mass spectrometry. These results suggest that sp. Q67 is a promising alternative for the rapid evaluation of the toxicity of fungal mycotoxins.
大多数热带水果在收获后很容易受到真菌感染而腐烂。由于一些病原真菌会产生有害化合物，如霉菌毒素，因此急需开发新型快速有效的方法来评估这些有害化合物。在这里，我们报告了发光细菌 sp. Q67 可用于快速评估霉菌毒素和产毒真菌培养物的毒性。毒素（伏马菌素 B1、脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮、赭曲霉毒素 A、展青霉素和桔青霉素）浓度与 sp. Q67 的发光强度之间存在很好的相关性（²＞0.98）。此外，还观察到 10 种主要产毒真菌培养物中的毒素含量与发光强度之间存在显著相关性（²＞0.96）。此外，与（IC = 92.56%）或（IC = 28.61%）相比，（半最大抑制浓度（IC）= 17.49%）表现出更强的发光抑制作用，这与高效液相色谱-串联质谱法测定的伏马菌素 B1、伏马菌素 B2 和脱氧雪腐镰刀菌烯醇的现有较高水平一致。这些结果表明，sp. Q67 是一种快速评估真菌霉菌毒素毒性的有前途的替代方法。
