Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.
Sensors (Basel). 2017 Oct 21;17(10):2410. doi: 10.3390/s17102410.
A new single-cell microarray chip was designed and developed to separate and analyze single adherent and non-adherent cancer cells. The single-cell microarray chip is made of polystyrene with over 60,000 microchambers of 10 different size patterns (31-40 µm upper diameter, 11-20 µm lower diameter). A drop of suspension of adherent carcinoma (NCI-H1650) and non-adherent leukocyte (CCRF-CEM) cells was placed onto the chip, and single-cell occupancy of NCI-H1650 and CCRF-CEM was determined to be 79% and 84%, respectively. This was achieved by controlling the chip design and surface treatment. Analysis of protein expression in single NCI-H1650 and CCRF-CEM cells was performed on the single-cell microarray chip by multi-antibody staining. Additionally, with this system, we retrieved positive single cells from the microchambers by a micromanipulator. Thus, this system demonstrates the potential for easy and accurate separation and analysis of various types of single cells.
设计并开发了一种新型的单细胞微阵列芯片，用于分离和分析单个贴壁和非贴壁癌细胞。该单细胞微阵列芯片由聚苯乙烯制成，具有 60,000 多个具有 10 种不同尺寸图案的微腔（上部直径为 31-40 µm，下部直径为 11-20 µm）。将一滴悬浮的贴壁癌细胞（NCI-H1650）和非贴壁白细胞（CCRF-CEM）细胞置于芯片上，分别确定 NCI-H1650 和 CCRF-CEM 的单细胞占有率为 79%和 84%。这是通过控制芯片设计和表面处理来实现的。通过多抗体染色，在单细胞微阵列芯片上对单个 NCI-H1650 和 CCRF-CEM 细胞中的蛋白质表达进行分析。此外，我们还通过微操作器从微腔中检索到阳性单细胞。因此，该系统展示了轻松、准确地分离和分析各种类型单细胞的潜力。
