State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, People's Republic of China.
Anal Chem. 2017 Nov 21;89(22):12115-12122. doi: 10.1021/acs.analchem.7b02709. Epub 2017 Nov 3.
Accurate measurement of intracellular temperature is of great significance in biology and medicine. With use of DNA nanotechnology and inspiration by nature's examples of "protective and reversible responses" exoskeletons, a scallop-inspired DNA nanomachine (SDN) is desgined as a ratiometric nanothermometer for intracellular temperature sensing. The SDN is composed of a rigid DNA tetrahedron, where a thermal-sensitive molecular beacon (MB) is embedded in one edge of the DNA tetrahedron. Relying on the thermal-sensitive MB and fluorescence resonance energy transfer (FRET) signaling mechanism, the "On" to "Off" signal is reversibly responding to "below" and "over" the melting temperature. Mimicking the functional anatomy of a scallop, the SDN exhibits high cellular permeability and resistance to enzymatic degradation, good reversibility, and tunable response range. Furthermore, FRET ratiometric signal that allows the simultaneous recording of two emission intensities at different wavelengths can provide a feasible approach for precise detection, minimizing the effect of system fluctuations.
准确测量细胞内温度在生物学和医学中具有重要意义。受 DNA 纳米技术的启发，并借鉴自然界“保护和可逆响应”外骨骼的实例，设计了一种基于扇贝启发的 DNA 纳米机器（SDN）作为用于细胞内温度感应的比率型纳米温度计。SDN 由刚性 DNA 四面体组成，其中一个热敏分子信标（MB）嵌入在 DNA 四面体的一个边缘上。依赖于热敏 MB 和荧光共振能量转移（FRET）信号机制，“开”到“关”信号对“低于”和“高于”融解温度呈可逆响应。仿生扇贝的功能解剖结构，SDN 表现出高细胞通透性和抗酶降解能力、良好的可逆性和可调的响应范围。此外，FRET 比率信号允许同时记录两个不同波长的发射强度，为精确检测提供了一种可行的方法，最大限度地减少了系统波动的影响。
