Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China.
Biosens Bioelectron. 2022 Nov 15;216:114627. doi: 10.1016/j.bios.2022.114627. Epub 2022 Aug 11.
Precise sensing of cellular temperature is one significant yet challenge task for studying miscellaneous biological processes. Herein, we report a light-controlled DNA nanothermometer that allow for real-time thermal sensing in extracellular microscope with high spatiotemporal resolution. The light-controlled DNA nanothermometer three key elements: a thermal-sensitive molecular beacon (MB) labelled with fluorophore Cy5 and Cy3 at its 5' and 3' termini, an inhibitor strand containing two photocleavable linkers (pc-linker), and a biotin modified strand, which could modify this three-strand hybridization complex onto the cell surface. Upon exposing to UV light irradiation, the light-controlled DNA nanothermometer could be remotely activated and enable to perform highly sensitive and practical ratiometric temperature sensing. Meanwhile, the light-controlled DNA nanothermometer could conduct temperature sensing in the extracellular microscope and demonstrates desirable sensitivity, excellent reversibility, and quantitative ability for extracellular temperature measurement. Therefore, this light-controlled DNA can serve as a promising tool for elucidating thermal-related cell physiological and pathological processes.
精确感知细胞温度是研究各种生物过程的一项重要但具有挑战性的任务。在这里，我们报告了一种光控 DNA 纳米温度计，它允许在具有高时空分辨率的细胞外显微镜中进行实时热感应。光控 DNA 纳米温度计有三个关键要素：一个热敏感的分子信标（MB），其 5'和 3'末端标记有荧光团 Cy5 和 Cy3，一个含有两个光裂解连接物（pc-linker）的抑制剂链，以及一个生物素修饰的链，它可以将这个三链杂交复合物修饰到细胞表面。在暴露于紫外光照射下，光控 DNA 纳米温度计可以被远程激活，并能够进行高度敏感和实用的比率温度感应。同时，光控 DNA 纳米温度计可以在细胞外显微镜中进行温度感应，表现出良好的灵敏度、优异的可逆性和定量能力，用于细胞外温度测量。因此，这种光控 DNA 可以作为一种有前途的工具，用于阐明与热相关的细胞生理和病理过程。
