一种基于DNA纳米线的局部催化发夹组装反应用于活细胞中微小RNA成像

Wei Qiaomei, Huang Jin, Li Jing, Wang Jiaoli, Yang Xiaohai, Liu Jianbo, Wang Kemin

State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province , Hunan University , Changsha , P. R. China . Email:

Chem Sci. 2018 Aug 20;9(40):7802-7808. doi: 10.1039/c8sc02943a. eCollection 2018 Oct 28.

Here, we have developed a localized catalytic hairpin assembly (LCHA) strategy for intracellular miR-21 imaging by using DNA nanowires confining both hairpin probes in a compact space. The LCHA is constructed by interval hybridization of DNA hairpin probe pairs to a DNA nanowire with multiplex footholds generated by alternating chain hybridization. Compared to the conventional catalytic hairpin assembly (CHA) strategy, the LCHA significantly shortens the reaction time and enhances the sensitivity. Moreover, the proposed LCHA can serve as a carrier for delivery of probes into live cells as well as protect the probes from nuclease degradation and enhances the stability. We anticipate that this design can be widely applied in facilitating basic biomedical research and disease diagnosis.

在此，我们开发了一种局部催化发夹组装（LCHA）策略，用于细胞内miR-21成像，该策略使用DNA纳米线将两个发夹探针限制在一个紧凑的空间内。LCHA是通过将DNA发夹探针与具有交替链杂交产生的多个立足点的DNA纳米线进行间隔杂交构建而成。与传统的催化发夹组装（CHA）策略相比，LCHA显著缩短了反应时间并提高了灵敏度。此外，所提出的LCHA可以作为将探针递送至活细胞的载体，同时保护探针免受核酸酶降解并提高稳定性。我们预计这种设计可广泛应用于促进基础生物医学研究和疾病诊断。