用某些L-γ-谷氨酰羧基苯胺比色法测定血清γ-谷氨酰转移酶活性
Colorimetric method for assay of serum gamma-glutamyltransferase activity with some L-gamma-glutamyl-carboxyanilides.
作者信息
Szewczuk A, Kuropatwa M, Lang D
机构信息
Biochemical Laboratory, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocøaw.
出版信息
Clin Chim Acta. 1988 Nov;178(1):35-40. doi: 10.1016/0009-8981(88)90266-5.
A simple method for the assay of serum gamma-glutamyltransferase activity with soluble L-gamma-glutamyl-carboxyanilides is presented. It is based on transformation of aminobenzoic acid liberated by the enzyme into colour complex with 4-dimethyl-aminobenzaldehyde or with 4-dimethylaminocinnamaldehyde in acid medium. Among three carboxyanilides studied the highest enzyme activity was noted with L-gamma-glutamyl-3-carboxyanilide and no activity with L-gamma-glutamyl-2-carboxyanilide. A good correlation was demonstrated for serum gamma-glutamyltransferase activity determined by the new colorimetric method and by the standard one with L-gamma-glutamyl-3-carboxy-4-nitroanilide.
介绍了一种用可溶性L-γ-谷氨酰羧基苯胺测定血清γ-谷氨酰转移酶活性的简单方法。该方法基于酶释放的氨基苯甲酸在酸性介质中与4-二甲基氨基苯甲醛或4-二甲基氨基肉桂醛转化为显色复合物。在所研究的三种羧基苯胺中，L-γ-谷氨酰-3-羧基苯胺的酶活性最高，而L-γ-谷氨酰-2-羧基苯胺无活性。新的比色法和用L-γ-谷氨酰-3-羧基-4-硝基苯胺的标准方法测定的血清γ-谷氨酰转移酶活性之间显示出良好的相关性。
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