对来自健康个体、患者、污水污泥、牛、鸡和生肉中产超广谱β-内酰胺酶菌株的表型特征分析。
Phenotypic characterization of extended-spectrum-beta-lactamase producing from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat.
作者信息
Saleem Rabia, Ejaz Hasan, Zafar Aizza, Younas Sonia, Rathore Ahsan Waheed
机构信息
Rabia Saleem, M.Phil Scholar, Department of Microbiology, The Children's Hospital and The Institute of Child Health, Lahore, Pakistan.
Hasan Ejaz, M.Phil, PhD. Department of Microbiology, The Children's Hospital and The Institute of Child Health, Lahore, Pakistan.
出版信息
Pak J Med Sci. 2017 Jul-Aug;33(4):886-890. doi: 10.12669/pjms.334.12647.
OBJECTIVE
The present study aimed to determine the frequency and antimicrobial profile of ESBL-producing isolates of in different environments.
METHODS
This cross-sectional study was conducted at The Children's Hospital and The Institute of Child Health, Lahore from July to December 2015. The faecal specimens from healthy individuals, patients, sewage sludge, cattle, chickens and raw meat (n = 122) were processed for microbiological analysis using MacConkey agar supplemented with cefotaxime. The identification of organisms was confirmed by API 10S and antimicrobial resistance profile was recorded by Kirby-Bauer disc diffusion method.
RESULTS
On the basis of screening, 77 (63.0%) specimens were found to be positive for ESBL production. The confirmation of 74 (60.0%) ESBL producing was done using double disc synergy test (DDST). The frequency of ESBL producing was found to be 17 (57.0%) in healthy individuals, 15 (53.0%) in patients, 10 (66.0%) in cattle faeces, 5 (71.0%) in sewage sludge, 14 (70.0%) in raw meat and 13 (59.0%) in chicken faeces. All of these isolates were resistant to cephalosporins and some of these were resistant to fluoroquinolones and meropenem. None of the isolates showed resistance to cefoperazone-sulbactam, imipenem, piperacillin-tazobactam and amikacin.
CONCLUSION
The prevalence of ESBL-producing was recorded in all the environments, suggesting a global expansion of these enzymes.
目的
本研究旨在确定不同环境中产超广谱β-内酰胺酶（ESBL）菌株的频率及其抗菌谱。
方法
本横断面研究于2015年7月至12月在拉合尔儿童医院和儿童健康研究所进行。对来自健康个体、患者、污水污泥、牛、鸡和生肉的粪便标本（n = 122），使用补充了头孢噻肟的麦康凯琼脂进行微生物分析。通过API 10S鉴定菌株，并采用 Kirby-Bauer 纸片扩散法记录抗菌药物耐药谱。
结果
经筛选，77份（63.0%）标本被发现产ESBL呈阳性。使用双纸片协同试验（DDST）对74份（60.0%）产ESBL菌株进行了确认。结果发现，健康个体中产ESBL菌株的频率为17例（57.0%），患者中为15例（53.0%），牛粪中为10例（66.0%），污水污泥中为5例（71.0%），生肉中为14例（70.0%），鸡粪中为13例（59.0%）。所有这些分离株均对头孢菌素耐药，其中一些对氟喹诺酮类和美罗培南耐药。没有分离株对头孢哌酮-舒巴坦、亚胺培南、哌拉西林-他唑巴坦和阿米卡星耐药。
结论
在所有环境中均记录到产ESBL菌株的流行情况，表明这些酶在全球范围内扩散。
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