Guangzhou Medical University, Guangzhou 510095, Guangdong Province, China.
Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen 518035, Guangdong Province, China.
Cell Biol Int. 2018 Mar;42(3):262-271. doi: 10.1002/cbin.10903. Epub 2017 Dec 20.
Mesenchymal stem cells (MSCs) are the primary source of cells used for cell-based therapy in tissue engineering. MSCs are found in synovial fluid, a source that could be conveniently used for cartilage tissue engineering. However, the purification and characterization of SF-MSCs has been poorly documented in the literature. Here, we outline an easy-to-perform approach for the isolation and culture of MSCs derived from human synovial fluid (hSF-MSCs). We have successfully purified hSF-MSCs using magnetic-activated cell sorting (MACS) using the MSC surface marker, CD90. Purified SF-MSCs demonstrate significant renewal capacity following several passages in culture. Furthermore, we demonstrated that MACS-sorted CD90 cells could differentiated into osteoblasts, adipocytes, and chondrocytes in vitro. In addition, we show that these cells can generate cartilage tissue in micromass culture as well. This study demonstrates that MACS is a useful tool that can be used for the purification of hSF-MSCs from synovial fluid. The proliferation properties and ability to differentiate into chondrocytes make these hSF-MSCs a promising source of stem cells for applications in cartilage repair.
间充质干细胞（MSCs）是组织工程中细胞治疗的主要细胞来源。MSCs 存在于滑液中，这是一种用于软骨组织工程的方便来源。然而，SF-MSCs 的纯化和表征在文献中记录甚少。在这里，我们概述了一种从人滑膜液中分离和培养间充质干细胞（hSF-MSCs）的简便方法。我们使用磁激活细胞分选（MACS），利用 MSC 表面标志物 CD90，成功地从 SF 中纯化了 hSF-MSCs。纯化的 SF-MSCs 在传代培养后表现出显著的更新能力。此外，我们证明 MACS 分选的 CD90 细胞可以在体外分化为成骨细胞、脂肪细胞和成软骨细胞。此外，我们还表明这些细胞可以在微团培养中产生软骨组织。这项研究表明，MACS 是一种有用的工具，可用于从滑膜液中纯化 hSF-MSCs。这些 hSF-MSCs 的增殖特性和向软骨细胞分化的能力使其成为软骨修复应用中干细胞的有前途来源。
