从兔模型中分离和纯化滑膜液来源间充质干细胞的磁激活细胞分选策略
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model.
作者信息
Jia Zhaofeng, Liang Yujie, Li Xingfu, Xu Xiao, Xiong Jianyi, Wang Daping, Duan Li
机构信息
Postgraduate institution, Guangzhou Medical University; Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology; Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopaedic Engineering, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University).
Department of Chemistry, Chinese University of Hong Kong; Shenzhen Kangning Hospital, Shenzhen Mental Health Center.
出版信息
J Vis Exp. 2018 Aug 10(138):57466. doi: 10.3791/57466.
Mesenchymal stem cells (MSCs) are the main cell source for cell-based therapy. MSCs from articular cavity synovial fluid could potentially be used for cartilage tissue engineering. MSCs from synovial fluid (SF-MSCs) have been considered promising candidates for articular regeneration, and their potential therapeutic benefit has made them an important research topic of late. SF-MSCs from the knee cavity of the New Zealand white rabbit can be employed as an optimized translational model to assess human regenerative medicine. By means of CD90-based magnetic activated cell sorting (MACS) technologies, this protocol successfully obtains rabbit SF-MSCs (rbSF-MSCs) from this rabbit model and further fully demonstrates the MSC phenotype of these cells by inducing them to differentiate to osteoblasts, adipocytes, and chondrocytes. Therefore, this approach can be applied in cell biology research and tissue engineering using simple equipment and procedures.
间充质干细胞（MSCs）是基于细胞治疗的主要细胞来源。来自关节腔滑液的MSCs有可能用于软骨组织工程。来自滑液的MSCs（SF-MSCs）被认为是关节再生的有前景的候选细胞，其潜在的治疗益处使其成为近期重要的研究课题。来自新西兰白兔膝关节腔的SF-MSCs可作为评估人类再生医学的优化转化模型。通过基于CD90的磁性激活细胞分选（MACS）技术，本方案成功地从该兔模型中获得兔SF-MSCs（rbSF-MSCs），并通过诱导它们分化为成骨细胞、脂肪细胞和软骨细胞进一步充分证明了这些细胞的MSC表型。因此，这种方法可以使用简单的设备和程序应用于细胞生物学研究和组织工程。
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