State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P. R. China.
Chemistry. 2018 Apr 17;24(22):5707-5722. doi: 10.1002/chem.201704349. Epub 2017 Dec 13.
Biocompatible reactions have emerged as versatile tools to build various molecular imaging probes that hold great promise for the detection of biological processes in vitro and/or in vivo. In this Minireview, we describe the recent advances in the development of a firefly luciferin-inspired biocompatible reaction between cyanobenzothiazole (CBT) and cysteine (Cys), and highlight its versatility to label proteins and build multimodality molecular imaging probes. The review starts from the general introduction of biocompatible reactions, which is followed by briefly describing the development of the firefly luciferin-inspired biocompatible chemistry. We then discuss its applications for the specific protein labeling and for the development of multimodality imaging probes (fluorescence, bioluminescence, MRI, PET, photoacoustic, etc.) that enable high sensitivity and spatial resolution imaging of redox environment, furin and caspase-3/7 activity in living cells and mice. Finally, we offer the conclusions and our perspective on the various and potential applications of this reaction. We hope that this review will contribute to the research of biocompatible reactions for their versatile applications in protein labeling and molecular imaging.
生物相容性反应已成为构建各种分子成像探针的通用工具，这些探针在体外和/或体内检测生物过程方面具有很大的应用潜力。在这篇综述中，我们描述了近发展起来的基于萤火虫荧光素的生物相容性反应，该反应在氰基苯并噻唑（CBT）和半胱氨酸（Cys）之间具有多功能性，可以标记蛋白质并构建多模态分子成像探针。综述首先从生物相容性反应的一般介绍开始，然后简要描述了基于萤火虫荧光素的生物相容性化学的发展。我们接着讨论了它在特定蛋白质标记和多模态成像探针（荧光、生物发光、MRI、PET、光声等）开发方面的应用，这些探针能够对活细胞和小鼠中的氧化还原环境、furin 和 caspase-3/7 活性进行高灵敏度和空间分辨率成像。最后，我们提供了对该反应的各种潜在应用的结论和展望。我们希望这篇综述将有助于生物相容性反应的研究，以促进其在蛋白质标记和分子成像中的广泛应用。
