用于生物发光成像的炔基虫荧光素类似物的设计与合成
Design and Synthesis of an Alkynyl Luciferin Analogue for Bioluminescence Imaging.
作者信息
Steinhardt Rachel C, O'Neill Jessica M, Rathbun Colin M, McCutcheon David C, Paley Miranda A, Prescher Jennifer A
机构信息
Department of Chemistry, University of California, Irvine, Irvine, CA, USA.
Department of Molecular Biology & Biochemistry, University of California, Irvine, Irvine, CA, USA.
出版信息
Chemistry. 2016 Mar 7;22(11):3671-5. doi: 10.1002/chem.201503944. Epub 2016 Jan 19.
Abstract
Herein, the synthesis and characterization of an alkyne-modified luciferin is reported. This bioluminescent probe was accessed using C-H activation methodology and was found to be stable in solution and capable of light production with firefly luciferase. The luciferin analogue was also cell permeant and emitted more redshifted light than d-luciferin, the native luciferase substrate. Based on these features, the alkynyl luciferin will be useful for a variety of imaging applications.
摘要
在此，报道了一种炔烃修饰的荧光素的合成与表征。这种生物发光探针采用碳氢键活化方法制备，在溶液中稳定，能与萤火虫荧光素酶产生光。该荧光素类似物还具有细胞渗透性，且比天然荧光素酶底物d -荧光素发射出更多红移光。基于这些特性，炔基荧光素将可用于多种成像应用。
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