Zhu Yuechao, Zhang Xian, You Qidong, Jiang Zhengyu
State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China; Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China; Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
Bioorg Med Chem. 2022 Aug 15;68:116881. doi: 10.1016/j.bmc.2022.116881. Epub 2022 Jun 11.
Click chemistry is a hot topic in many research fields. A biocompatible reaction from fireflies has attracted increasing attention since 2009. Herein, we focus on the firefly-sourced click reaction between cysteine (Cys) and 2-cyanobenzothiazole (2-CBT). This reaction has many excellent properties, such as rapidity, simplicity and high selectivity, which make it successfully applied in protein labeling, molecular imaging, drug discovery and other fields. Meanwhile, its unique ability to form nanoparticles expands its applications in biological systems. We review its principle, development, and latest applications in the past 5 years and hope this review provides more profound and comprehensive insights to its further application.
点击化学是许多研究领域的热门话题。自2009年以来，一种源自萤火虫的生物相容性反应受到了越来越多的关注。在此，我们聚焦于半胱氨酸（Cys）与2-氰基苯并噻唑（2-CBT）之间源自萤火虫的点击反应。该反应具有许多优异的特性，如快速、简便和高选择性，这使其成功应用于蛋白质标记、分子成像、药物发现等领域。同时，其形成纳米颗粒的独特能力扩展了其在生物系统中的应用。我们综述了其原理、发展以及过去5年的最新应用，希望本综述能为其进一步应用提供更深刻和全面的见解。
