Laboratoire de Biotechnologie Appliquée à l'Agriculture, Institut National de la Recherche Agronomique de Tunisie (INRAT), Université de Carthage, El Rue Hedi Karray Menzah, 1004 Tunis, Tunisia.
Institute for Sustainable Plant Protection (IPSP), CNR, 10135 Turin, Italy.
Viruses. 2017 Oct 25;9(11):311. doi: 10.3390/v9110311.
(CpCDV), a polyphagous mastrevirus, family , has been recently linked to the onset of the "hard fruit syndrome" of watermelon, first described in Tunisia, that makes fruits unmarketable due to the presence of white hard portions in the flesh, chlorotic mottling on the rind, and an unpleasant taste. To investigate the etiological agent of this disease, total RNA extracted from symptomatic watermelon fruits was subjected to small RNA sequencing through next generation sequencing (NGS) techniques. Data obtained showed the presence of CpCDV and two other viral species. However, following validation through polymerase chain reaction (PCR), CpCDV was the only viral species consistently detected in all samples. Watermelon seedlings were then challenged by an agroinfectious CpCDV clone; several plants proved to be CpCDV-infected, and were able to produce fruits. CpCDV infected and replicated in watermelon fruits and leaves, leading to abnormality in fruits and in seed production, similar to those described in field. These results indicate that CpCDV is the etiological agent of the "hard fruit syndrome" of watermelon.
(CpCDV)，一种多食性的马斯特罗病毒，家族，最近与西瓜的“硬果综合征”有关，该综合征首先在突尼斯被描述，由于果肉中存在白色硬部分、果皮出现黄化斑驳和口感不佳，使果实变得无法销售。为了调查这种疾病的病因，从有症状的西瓜果实中提取总 RNA，通过下一代测序（NGS）技术进行小 RNA 测序。获得的数据显示存在 CpCDV 和另外两种病毒。然而，通过聚合酶链反应（PCR）验证后，CpCDV 是所有样本中唯一一致检测到的病毒。然后，用农杆菌感染性 CpCDV 克隆对西瓜幼苗进行了挑战；一些植物被证明感染了 CpCDV，并能够产生果实。CpCDV 感染和复制在西瓜果实和叶子中，导致果实和种子生产异常，与田间描述的相似。这些结果表明，CpCDV 是西瓜“硬果综合征”的病原体。
