Kraberger Simona, Kumari Safaa G, Hamed Abdelmagid A, Gronenborn Bruno, Thomas John E, Sharman Murray, Harkins Gordon W, Muhire Brejnev M, Martin Darren P, Varsani Arvind
School of Biological Sciences and Biomolecular Interaction Centre, University of Canterbury, Christchurch 8140, New Zealand.
Virology Laboratory, International Centre for Agricultural Research in the Dry Areas (ICARDA), Tunis, Tunisia.
Infect Genet Evol. 2015 Jan;29:203-15. doi: 10.1016/j.meegid.2014.11.024. Epub 2014 Nov 29.
In Sudan Chickpea chlorotic dwarf virus (CpCDV, genus Mastrevirus, family Geminiviridae) is an important pathogen of pulses that are grown both for local consumption, and for export. Although a few studies have characterised CpCDV genomes from countries in the Middle East, Africa and the Indian subcontinent, little is known about CpCDV diversity in any of the major chickpea production areas in these regions. Here we analyse the diversity of 146 CpCDV isolates characterised from pulses collected across the chickpea growing regions of Sudan. Although we find that seven of the twelve known CpCDV strains are present within the country, strain CpCDV-H alone accounted for ∼73% of the infections analysed. Additionally we identified four new strains (CpCDV-M, -N, -O and -P) and show that recombination has played a significant role in the diversification of CpCDV, at least in this region. Accounting for observed recombination events, we use the large amounts of data generated here to compare patterns of natural selection within protein coding regions of CpCDV and other dicot-infecting mastrevirus species.
在苏丹，鹰嘴豆褪绿矮缩病毒（CpCDV，属于 Mastrevirus 属，双生病毒科）是豆类作物的一种重要病原体，这些豆类既用于当地消费，也用于出口。尽管有一些研究对来自中东、非洲和印度次大陆国家的 CpCDV 基因组进行了特征描述，但对于这些地区任何一个主要鹰嘴豆生产区的 CpCDV 多样性却知之甚少。在此，我们分析了从苏丹鹰嘴豆种植区收集的豆类中鉴定出的 146 株 CpCDV 分离株的多样性。尽管我们发现该国存在已知的 12 种 CpCDV 菌株中的 7 种，但仅 CpCDV-H 菌株就占所分析感染的约 73%。此外，我们鉴定出了 4 种新菌株（CpCDV-M、-N、-O 和 -P），并表明重组至少在该地区 CpCDV 的多样化过程中发挥了重要作用。考虑到观察到的重组事件，我们利用此处生成的大量数据来比较 CpCDV 和其他感染双子叶植物的 Mastrevirus 属病毒物种蛋白质编码区域内的自然选择模式。
