Romuk Ewa B, Szczurek Wioletta, Oleś Michał, Gabrysiak Artur, Skowron Marta, Nowak Przemysław, Birkner Ewa
Department of Biochemistry, Medical University of Silesia, School of Medicine with the Division of Dentistry, Zabrze, Poland.
Department of Toxicology and Health Protection, Medical University of Silesia, School of Public Health, Katowice, Poland.
Adv Clin Exp Med. 2017 Sep;26(6):953-959. doi: 10.17219/acem/63999.
Parkinson's disease is a progressive neurodegenerative disorder, characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta. The causes of Parkinson's disease are not fully understood; however, increasing evidence implicates oxidative stress.
The study was aimed at assessing the nature of the changes in the oxidation-antioxidant balance in the cerebral cortex, striatum, hippocampus, thalamus, and cerebellum in a rat model of Parkinson's disease (PD).
Sixteen male Wistar rats were divided into 2 groups: Icontrol, IIParkinson's disease. The 8-weeks-old animals were decapitated, their brains removed and the following structures dissected and then frozen for further biochemical assays: cerebral cortex, striatum, hippocampus, thalamus and cerebellum. The activities of: the catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione S-transferase (GST), superoxide dismutase (SOD) and the isoenzymes: Cu/ZnSOD and MnSOD; together with the malondialdehyde (MDA) and the total oxidative status (TOS) concentrations were measured in each structure.
A significantly increased activities of SOD, Cu/ZnSOD, GST and reduced GR activity and an increase of MDA concentration were observed in the striatum of PD rats, comparing to the control group, combined with a significantly reduced activities of GR,SOD, Cu/ZnSOD and an increased GPX activity and MDA concentration in the hippocampus, a significantly lower GR, SOD, MnSOD, Cu/ZnSOD, and GST activities in the cerebral cortex. A significantly lower GR activity, higher CAT activity and MDA concentration in the thalamus and a significantly increased GR activity in the cerebellum were observed in PD rats compared to the corresponding control group.
Oxidative stress in PD involves many brain structures and various antioxidant enzymes and oxidative status parameters become dysfunctional, depending on the area of the brain, which might reflect the complexity of the clinical symptoms of PD.
帕金森病是一种进行性神经退行性疾病，其特征是黑质致密部多巴胺能神经元的丧失。帕金森病的病因尚未完全明确；然而，越来越多的证据表明氧化应激与之相关。
本研究旨在评估帕金森病（PD）大鼠模型大脑皮质、纹状体、海马、丘脑和小脑中氧化-抗氧化平衡变化的性质。
16只雄性Wistar大鼠分为2组：I对照组，II帕金森病组。将8周龄的动物断头，取出大脑，解剖以下结构，然后冷冻以备进一步生化检测：大脑皮质、纹状体、海马、丘脑和小脑。测定各结构中过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S-转移酶（GST）、超氧化物歧化酶（SOD）及其同工酶铜/锌超氧化物歧化酶（Cu/ZnSOD）和锰超氧化物歧化酶（MnSOD）的活性，以及丙二醛（MDA）和总氧化状态（TOS）的浓度。
与对照组相比，PD大鼠纹状体中SOD、Cu/ZnSOD、GST活性显著增加，GR活性降低，MDA浓度升高；海马中GR、SOD、Cu/ZnSOD活性显著降低，GPX活性和MDA浓度升高；大脑皮质中GR、SOD、MnSOD、Cu/ZnSOD和GST活性显著降低。与相应对照组相比，PD大鼠丘脑中GR活性显著降低，CAT活性和MDA浓度升高，小脑中GR活性显著升高。
PD中的氧化应激涉及多个脑结构，各种抗氧化酶和氧化状态参数出现功能障碍，这取决于脑区，这可能反映了PD临床症状的复杂性。
