Patthy M, Gyenge R
Institute for Drug Research, Budapest, Hungary.
J Chromatogr. 1988 Sep 30;449(1):191-205. doi: 10.1016/s0021-9673(00)94379-6.
The behaviour of trifluoroacetate and heptafluorobutyrate as pairing ions for the reversed-phase ion-pair separation of monoamine transmitters and related metabolites was studied. The performance of systems with the perfluorinated acids was compared with that of systems containing sodium octyl sulphonate and was found to be better in terms of peak resolution combined with total analysis time, day-to-day reproducibility and the time required for attaining initial chromatographic equilibrium. Rat brain samples were deproteinized in the acidified mobile phase, injected directly on to a high-performance liquid chromatographic column and quantitated using an amperometric detector. Sample run times were 6-8 min, at a relatively low flow-rate. The detection limits achieved are fairly uncommon with conventional bore columns. The two perfluorinated acids studied differ in the dominant mechanisms of ion-pair formation and show selectivity differences as a result.
研究了三氟乙酸盐和七氟丁酸盐作为单胺类递质及其相关代谢产物反相离子对分离的配对离子的行为。将含全氟代酸的系统性能与含辛基磺酸钠的系统性能进行了比较，发现在峰分辨率、总分析时间、日常重现性以及达到初始色谱平衡所需时间方面，前者表现更佳。大鼠脑样品在酸化流动相中进行脱蛋白处理，直接进样到高效液相色谱柱上，并使用安培检测器进行定量分析。样品运行时间为6 - 8分钟，流速相对较低。所实现的检测限在常规内径柱中相当少见。所研究的两种全氟代酸在离子对形成的主导机制上有所不同，因此表现出选择性差异。
