Durkin T A, Anderson G M, Cohen D J
Child Study Center, Yale University School of Medicine, New Haven, CT 06510.
J Chromatogr. 1988 Jun 24;428(1):9-15. doi: 10.1016/s0378-4347(00)83885-2.
Rapid and selective determination of neurotransmitter amino acids in rat and human brain is accomplished by pre-column derivatization with o-phthaldehyde-3-mercaptopropionic acid, reversed-phase high-performance liquid chromatographic separation, and fluorimetric detection. Aspartate, taurine, glutamate, and glycine are determined in less than 12 min with intra- and inter-assay precisions of 2.1-9.5% and 4.2-9.2%, respectively. gamma-Aminobutyric acid is determined in less than 8 min with intra- and inter-assay precisions of 1.6 and 11.7%, respectively. Both assays utilize internal standards and require a minimum of sample preparation.
通过邻苯二甲醛-3-巯基丙酸进行柱前衍生、反相高效液相色谱分离和荧光检测，可快速、选择性地测定大鼠和人类大脑中的神经递质氨基酸。天冬氨酸、牛磺酸、谷氨酸和甘氨酸在不到12分钟内即可测定，批内和批间精密度分别为2.1%-9.5%和4.2%-9.2%。γ-氨基丁酸在不到8分钟内即可测定，批内和批间精密度分别为1.6%和11.7%。两种测定方法均使用内标，且所需样品制备最少。
