Nakagawa Hiroyuki, Matsuo Yosuke, McCormick Susan, Lim Chee Wei
National Agriculture and Food Research Organization, Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan.
U.S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N University St, Peoria, IL 61604.
J AOAC Int. 2018 May 1;101(3):658-666. doi: 10.5740/jaoacint.17-0362. Epub 2017 Oct 25.
A determination method previously validated for trichothecenes and zearalenone by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS) was adapted for the quantification of T-2 toxin (T-2) as well as its glucoside and acetyl derivatives, T-2-3-glucoside (T-2-3G) and 3-acetyl-T-2 (3A-T-2). HT-2 toxin (HT-2) and its acetyl derivative 3-acetyl-HT-2 (3A-HT-2) were also included as the target chemicals. Staple flours (56 samples collected from the Singapore market) were examined for contamination from T-2 and/or HT-2 and their derivatives. Among them, 16 flours were found to be contaminated with T-2 and/or HT-2, whereas none was contaminated with T-2-3G and 3A-HT-2, except for trace 3A-T-2 detected in 2 rye samples. Rye flour samples were frequently contaminated with both T-2 and HT-2. Some of the reference materials (RMs) were further analyzed, and T-2-3G and 3A-T-2 were quantitatively detected in corn and wheat RMs. The ratio of T-2-3G to T-2 in the RMs seemed to be much lower than the ratio of deoxynivalenol-3-glucoside to deoxynivalenol usually reported in former studies. To the best of our knowledge, the natural contamination of 3A-T-2 in staple flour is reported here for the first time.
一种先前已通过液相色谱 - 串联质谱法（LC - MS/MS）验证用于测定单端孢霉烯族毒素和玉米赤霉烯酮的测定方法，被应用于T - 2毒素（T - 2）及其糖苷和乙酰基衍生物T - 2 - 3 - 葡糖苷（T - 2 - 3G）和3 - 乙酰基 - T - 2（3A - T - 2）的定量分析。HT - 2毒素（HT - 2）及其乙酰基衍生物3 - 乙酰基 - HT - 2（3A - HT - 2）也被列为目标化学物质。对主食面粉（从新加坡市场收集的56个样品）进行了T - 2和/或HT - 2及其衍生物污染情况的检测。其中，16种面粉被检测出受到T - 2和/或HT - 2污染，而除了在2个黑麦样品中检测到痕量的3A - T - 2外，没有样品被T - 2 - 3G和3A - HT - 2污染。黑麦面粉样品经常同时受到T - 2和HT - 2污染。对一些参考物质（RMs）进行了进一步分析，在玉米和小麦RMs中定量检测到了T - 2 - 3G和3A - T - 2。参考物质中T - 2 - 3G与T - 2的比例似乎远低于以往研究中通常报道的脱氧雪腐镰刀菌烯醇 - 3 - 葡糖苷与脱氧雪腐镰刀菌烯醇的比例。据我们所知，本文首次报道了主食面粉中3A - T - 2的天然污染情况。
