U.S. Agricultural Research Service, National Center for Agricultural Utilization Department of Agriculture Research, Peoria, IL 61604, USA.
Toxins (Basel). 2011 Dec;3(12):1554-68. doi: 10.3390/toxins3121554. Epub 2011 Dec 20.
The trichothecenes produced by solid and liquid cultures of Fusarium sporotrichioides were evaluated with high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). Along with the expected T-2 toxin HT-2 toxin and neosolaniol, two additional compounds were detected, which had ions 162 m/z higher than those in the mass spectra of T-2 toxin or HT-2 toxin. Fragmentation behavior of these two compounds was similar to that of T-2 toxin and HT-2 toxin. Based on LC-MS/MS behavior, it is proposed that the two compounds are T-2 toxin 3-O-glucoside and HT-2 toxin 3-O-glucoside. Production of the two glucosides was measured in kernels from wheat and oat inoculated with F. sporotrichiodes, as well as in cultures grown in liquid media and on cracked corn or rice. Production of glucosides in wheat and oats suggest that they may also be present in naturally contaminated cereals.
采用高效液相色谱-串联质谱法（LC-MS/MS）评估了木贼镰孢固态和液态培养物产生的单端孢霉烯族化合物。除了预期的 T-2 毒素、HT-2 毒素和新茄病镰刀菌醇外，还检测到两种另外的化合物，其离子质荷比（m/z）比 T-2 毒素或 HT-2 毒素的质谱中的离子质荷比高 162。这两种化合物的碎裂行为与 T-2 毒素和 HT-2 毒素的碎裂行为相似。根据 LC-MS/MS 行为，这两种化合物被提议为 T-2 毒素 3-O-葡萄糖苷和 HT-2 毒素 3-O-葡萄糖苷。用木贼镰孢接种的小麦和燕麦的籽粒以及在液体培养基和粉碎玉米或大米上生长的培养物中测量了这两种葡萄糖苷的产生。在小麦和燕麦中产生葡萄糖苷表明它们也可能存在于受污染的天然谷物中。
