• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-204 与人类胰岛中的内分泌表型有关，但不通过 MAFA 调节胰岛素 mRNA。

miR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA.

机构信息

Diabetes Research Institute, Division of Immunology, Transplantation and Infectious Disease, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Human Pathologies Genomic Diagnostics unit, Division of Genetics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy.

出版信息

Sci Rep. 2017 Oct 25;7(1):14051. doi: 10.1038/s41598-017-13622-7.

DOI:10.1038/s41598-017-13622-7
PMID:29070792
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5656581/
Abstract

miR-204 has been proposed to modulate insulin expression in human pancreatic islets by regulating the expression of the MAFA transcript, and in turn insulin transcription. We investigated miR-204 expression in pancreatic endocrine tumors (PET), a panel of human tissues, tissues derived from pancreatic islet purification, and in induced pluripotent stem cells (iPSCs) differentiated towards a pancreatic endocrine phenotype by quantitative real time RT-PCR or droplet digital PCR (ddPCR). In addition, we evaluated the effect of miR-204 up- or down-regulation in purified human islets and in the EndoC-βH1 cell line, as an experimental model of human pancreatic β cells. Our results confirm that miR-204 was enriched in insulin producing PET, in β cells within healthy pancreatic islets, and highly expressed in EndoC-βH1 cells. Moreover, in iPSCs miR-204 increased stepwise upon stimulated differentiation to insulin producing cells. However, up- or down-regulation of miR-204 in human islets and in EndoC-βH1 cells resulted in modest and not significant changes of the MAFA and INS mRNAs measured by ddPCR or c-peptide release. Our data confirm the association of miR-204 with a β cell endocrine phenotype in human pancreatic islets, but do not support its direct role in regulating the levels of insulin mRNA through MAFA.

摘要

miR-204 被提出通过调节 MAFA 转录物的表达，进而调节胰岛素转录，来调节人胰腺胰岛中的胰岛素表达。我们通过定量实时 RT-PCR 或液滴数字 PCR（ddPCR）在胰腺内分泌肿瘤（PET）、一组人类组织、从胰腺胰岛纯化中获得的组织以及分化为胰腺内分泌表型的诱导多能干细胞（iPSC）中研究了 miR-204 的表达。此外，我们评估了 miR-204 在纯化的人胰岛和 EndoC-βH1 细胞系中的上调或下调对实验模型人胰腺β细胞的影响。我们的结果证实 miR-204 在产生胰岛素的 PET 中丰富，在健康胰腺胰岛中的β细胞中丰富，并且在 EndoC-βH1 细胞中高度表达。此外，在 iPSC 中，miR-204 在刺激分化为产生胰岛素的细胞时逐步增加。然而，在人胰岛和 EndoC-βH1 细胞中上调或下调 miR-204 导致 ddPCR 或 C 肽释放测量的 MAFA 和 INS mRNA 的变化适度且无统计学意义。我们的数据证实了 miR-204 与人胰腺胰岛中β细胞内分泌表型的关联，但不支持其通过 MAFA 直接调节胰岛素 mRNA 水平的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/c4cb42d0e6d3/41598_2017_13622_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/747ee69b00d4/41598_2017_13622_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/e4bc6ace0fcf/41598_2017_13622_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/365061169680/41598_2017_13622_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/10ff789e9d38/41598_2017_13622_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/15e582b8ebe4/41598_2017_13622_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/9ea16ada2ab3/41598_2017_13622_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/0072aba62424/41598_2017_13622_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/c4cb42d0e6d3/41598_2017_13622_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/747ee69b00d4/41598_2017_13622_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/e4bc6ace0fcf/41598_2017_13622_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/365061169680/41598_2017_13622_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/10ff789e9d38/41598_2017_13622_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/15e582b8ebe4/41598_2017_13622_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/9ea16ada2ab3/41598_2017_13622_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/0072aba62424/41598_2017_13622_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2559/5656581/c4cb42d0e6d3/41598_2017_13622_Fig8_HTML.jpg

相似文献

1
miR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA.miR-204 与人类胰岛中的内分泌表型有关，但不通过 MAFA 调节胰岛素 mRNA。
Sci Rep. 2017 Oct 25;7(1):14051. doi: 10.1038/s41598-017-13622-7.
2
A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells.从MafB到MafA表达的转变伴随着向胰腺β细胞的分化。
Dev Biol. 2006 May 15;293(2):526-39. doi: 10.1016/j.ydbio.2006.02.028. Epub 2006 Apr 3.
3
microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9.microRNA-690 通过靶向 Sox9 调节诱导多能干细胞（iPSCs）分化为胰岛素生成细胞。
Stem Cell Res Ther. 2019 Feb 15;10(1):59. doi: 10.1186/s13287-019-1154-8.
4
MafA promotes the reprogramming of placenta-derived multipotent stem cells into pancreatic islets-like and insulin+ cells.MafA 促进胎盘来源的多能干细胞重编程为胰岛样细胞和胰岛素+细胞。
J Cell Mol Med. 2011 Mar;15(3):612-24. doi: 10.1111/j.1582-4934.2010.01034.x.
5
MicroRNA expression profiles of human iPSCs differentiation into insulin-producing cells.人诱导多能干细胞分化为胰岛素分泌细胞过程中的微小RNA表达谱
Acta Diabetol. 2017 Mar;54(3):265-281. doi: 10.1007/s00592-016-0955-9. Epub 2016 Dec 30.
6
Differentiation of iPSCs into insulin-producing cells via adenoviral transfection of PDX-1, NeuroD1 and MafA.通过腺病毒转染PDX-1、NeuroD1和MafA将诱导多能干细胞分化为胰岛素分泌细胞。
Diabetes Res Clin Pract. 2014 Jun;104(3):383-92. doi: 10.1016/j.diabres.2014.03.017. Epub 2014 Apr 1.
7
MAFA and T3 Drive Maturation of Both Fetal Human Islets and Insulin-Producing Cells Differentiated From hESC.MAFA和T3驱动人胎儿胰岛以及从人胚胎干细胞分化而来的胰岛素生成细胞的成熟。
J Clin Endocrinol Metab. 2015 Oct;100(10):3651-9. doi: 10.1210/jc.2015-2632. Epub 2015 Jul 24.
8
MAFA and MAFB regulate exocytosis-related genes in human β-cells.MAFA和MAFB调节人类β细胞中与胞吐作用相关的基因。
Acta Physiol (Oxf). 2022 Feb;234(2):e13761. doi: 10.1111/apha.13761. Epub 2022 Jan 11.
9
Long Noncoding RNA Meg3 Regulates Mafa Expression in Mouse Beta Cells by Inactivating Rad21, Smc3 or Sin3α.长链非编码RNA Meg3通过使Rad21、Smc3或Sin3α失活来调节小鼠β细胞中的Mafa表达。
Cell Physiol Biochem. 2018;45(5):2031-2043. doi: 10.1159/000487983. Epub 2018 Mar 6.
10
MafA regulates expression of genes important to islet beta-cell function.MafA调节对胰岛β细胞功能至关重要的基因的表达。
Mol Endocrinol. 2007 Nov;21(11):2764-74. doi: 10.1210/me.2007-0028. Epub 2007 Jul 17.

引用本文的文献

1
The power of microRNA regulation-insights into immunity and metabolism.微小RNA调控的力量——对免疫和代谢的见解
FEBS Lett. 2025 Jul;599(13):1821-1851. doi: 10.1002/1873-3468.70039. Epub 2025 Apr 11.
2
Bioengineering of a human iPSC-derived vascularized endocrine pancreas for type 1 diabetes.用于1型糖尿病的人诱导多能干细胞衍生的血管化内分泌胰腺的生物工程
Cell Rep Med. 2025 Feb 18;6(2):101938. doi: 10.1016/j.xcrm.2025.101938. Epub 2025 Feb 7.
3
miRNAs in the Beta Cell-Friends or Foes?胰岛β细胞中的 microRNAs：朋友还是敌人？

本文引用的文献

1
Human induced pluripotent stem cells differentiate into insulin-producing cells able to engraft in vivo.人诱导多能干细胞分化为能够在体内移植的胰岛素生成细胞。
Acta Diabetol. 2015 Dec;52(6):1025-35. doi: 10.1007/s00592-015-0726-z. Epub 2015 Mar 4.
2
Human islet distribution programme for basic research: activity over the last 5 years.用于基础研究的人类胰岛分布计划：过去5年的活动情况
Diabetologia. 2015 May;58(5):1138-40. doi: 10.1007/s00125-015-3536-5. Epub 2015 Feb 21.
3
Re-thinking miRNA-mRNA interactions: intertwining issues confound target discovery.
Endocrinology. 2023 Mar 13;164(5). doi: 10.1210/endocr/bqad040.
4
Directed self-assembly of a xenogeneic vascularized endocrine pancreas for type 1 diabetes.异种血管化内分泌胰腺的定向自组装治疗 1 型糖尿病。
Nat Commun. 2023 Feb 16;14(1):878. doi: 10.1038/s41467-023-36582-1.
5
iPSC-Derived Pancreatic Progenitors Lacking FOXA2 Reveal Alterations in miRNA Expression Targeting Key Pancreatic Genes.iPSC 衍生的缺乏 FOXA2 的胰腺祖细胞揭示了 miRNA 表达对关键胰腺基因的靶向改变。
Stem Cell Rev Rep. 2023 May;19(4):1082-1097. doi: 10.1007/s12015-023-10515-3. Epub 2023 Feb 7.
6
miR204 potentially promotes non-alcoholic fatty liver disease by inhibition of cpt1a in mouse hepatocytes.miR204 通过抑制小鼠肝细胞中的 cpt1a 促进非酒精性脂肪性肝病。
Commun Biol. 2022 Sep 21;5(1):1002. doi: 10.1038/s42003-022-03945-1.
7
MafA Regulation in β-Cells: From Transcriptional to Post-Translational Mechanisms.MafA 在 β 细胞中的调控：从转录到翻译后机制。
Biomolecules. 2022 Mar 31;12(4):535. doi: 10.3390/biom12040535.
8
Tissue miR-200c-3p and circulating miR-1290 as potential prognostic biomarkers for colorectal cancer.组织 miR-200c-3p 和循环 miR-1290 作为结直肠癌潜在的预后生物标志物。
Sci Rep. 2022 Feb 10;12(1):2295. doi: 10.1038/s41598-022-06192-w.
9
Machine learning workflows identify a microRNA signature of insulin transcription in human tissues.机器学习工作流程确定了人类组织中胰岛素转录的微小RNA特征。
iScience. 2021 Mar 31;24(4):102379. doi: 10.1016/j.isci.2021.102379. eCollection 2021 Apr 23.
10
The Landscape of microRNAs in βCell: Between Phenotype Maintenance and Protection.β细胞中微小RNA的格局：介于表型维持与保护之间
Int J Mol Sci. 2021 Jan 14;22(2):803. doi: 10.3390/ijms22020803.
重新思考微小RNA与信使核糖核酸的相互作用：相互交织的问题困扰着靶标发现。
Bioessays. 2015 Apr;37(4):379-88. doi: 10.1002/bies.201400191. Epub 2015 Feb 12.
4
TRPM3 and miR-204 establish a regulatory circuit that controls oncogenic autophagy in clear cell renal cell carcinoma.瞬时受体电位阳离子通道亚家族M成员3（TRPM3）和微小RNA-204（miR-204）建立了一个调控环路，该环路可控制透明细胞肾细胞癌中的致癌性自噬。
Cancer Cell. 2014 Nov 10;26(5):738-53. doi: 10.1016/j.ccell.2014.09.015.
5
Absolute quantification by droplet digital PCR versus analog real-time PCR.通过液滴数字 PCR 进行绝对定量与模拟实时 PCR。
Nat Methods. 2013 Oct;10(10):1003-5. doi: 10.1038/nmeth.2633. Epub 2013 Sep 1.
6
Thioredoxin-interacting protein regulates insulin transcription through microRNA-204.硫氧还蛋白相互作用蛋白通过 microRNA-204 调节胰岛素转录。
Nat Med. 2013 Sep;19(9):1141-6. doi: 10.1038/nm.3287. Epub 2013 Aug 25.
7
MicroRNA expression in alpha and beta cells of human pancreatic islets.人胰腺胰岛中α和β细胞的 microRNA 表达。
PLoS One. 2013;8(1):e55064. doi: 10.1371/journal.pone.0055064. Epub 2013 Jan 29.
8
A genetically engineered human pancreatic β cell line exhibiting glucose-inducible insulin secretion.一种具有葡萄糖诱导胰岛素分泌功能的基因工程人胰腺β细胞系。
J Clin Invest. 2011 Sep;121(9):3589-97. doi: 10.1172/JCI58447. Epub 2011 Aug 25.
9
Transient receptor potential cation channels in pancreatic β cells.胰腺β细胞中的瞬时受体电位阳离子通道。
Rev Physiol Biochem Pharmacol. 2011;161:87-110. doi: 10.1007/112_2011_2.
10
Analytical aspects of microRNA in diagnostics: a review.miRNA 在诊断学中的分析方面：综述
Anal Chim Acta. 2011 Aug 12;699(2):134-52. doi: 10.1016/j.aca.2011.05.025. Epub 2011 May 20.