Wang Sheng, Weng Jian, Fu Xiao, Lin Jing, Fan Wenpei, Lu Nan, Qu JunLe, Chen Siping, Wang Tianfu, Huang Peng
Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen 518060, China.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Nanotheranostics. 2017 May 30;1(2):208-216. doi: 10.7150/ntno.18767. eCollection 2017.
Near-infrared (NIR) light triggered photothermally enhanced cellular internalization strategy shows great potential to improve antitumor effect to fight against cancer. In this work, a black phosphorus (BP) nanosheet-based nanomedicine, which is composed of BP nanosheet, human serum albumin (HSA) and paclitaxel (PTX), was developed for mild hyperthermia-enhanced intracellular drug delivery and chemo-photothermal combination therapy. BP nanosheet was successfully synthesized by a one-pot biomimetic synthesis method using HSA as exfoliating agent and capping agent. Meanwhile, PTX can be efficiently loaded by HSA via hydrophobic interactions. The PTX-loaded HSA functionalized BP (BP-HSA-PTX) exhibits great photothermal performance, excellent biodegradability/biocompatibility, and effective drug loading. Upon NIR laser illumination, mild hyperthermia (~43 °C) or photothermal effect can be achieved to improve the intracellular drug delivery efficiency and antitumor effect of BP-HSA-PTX. Our results indicate that HSA-functionalized BP has great potential for the loading, delivery, cellular uptake enhancement of chemotherapeutics and chemo-photothermal combination antitumor effect.
近红外（NIR）光触发的光热增强细胞内化策略在提高抗癌抗肿瘤效果方面显示出巨大潜力。在这项工作中，开发了一种基于黑磷（BP）纳米片的纳米药物，它由BP纳米片、人血清白蛋白（HSA）和紫杉醇（PTX）组成，用于温和热疗增强细胞内药物递送和化疗-光热联合治疗。以HSA作为剥离剂和封端剂，通过一锅法仿生合成成功制备了BP纳米片。同时，PTX可以通过疏水相互作用被HSA有效负载。负载PTX的HSA功能化BP（BP-HSA-PTX）表现出优异的光热性能、良好的生物降解性/生物相容性和有效的药物负载能力。在近红外激光照射下，可以实现温和热疗（约43°C）或光热效应，以提高BP-HSA-PTX的细胞内药物递送效率和抗肿瘤效果。我们的结果表明，HSA功能化BP在化疗药物的负载、递送、细胞摄取增强以及化疗-光热联合抗肿瘤效果方面具有巨大潜力。
