Bazazzadegan Niloofar, Dehghan Shasaltaneh Marzieh, Saliminejad Kioomars, Kamali Koorosh, Banan Mehdi, Khorram Khorshid Hamid Reza
Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Laboratory of Neuro-organic Chemistry, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
Adv Pharm Bull. 2017 Sep;7(3):491-494. doi: 10.15171/apb.2017.060. Epub 2017 Sep 25.
Sporadic Alzheimer's disease (AD) accounts for over 95% of cases. Possible mechanisms of AD such as inflammation and oxidative stresses in the brain motivate researchers to follow many therapies which would be effective, especially in the early stages of the disease. IMOD, the herbal extract of R. Canina, T. Vulgare and U. Dioica plant species enriched with selenium, has anti-inflammatory, immunoregulatory and protective effects against oxidative stress. In this study three AD-related genes, DAXX, NFκβ and VEGF, were chosen as candidate to investigate the neuroprotective effect of the extract by comparing their expression levels in the hippocampus of rat model of sporadic AD, using qPCR in the herbal-treated and control groups. The therapeutic effects on learning and memory levels were evaluated by Morris Water Maze (MWM) test. Gene expression results were indicative of significant up-regulation of Vegf in rat's hippocampus after treatment with the herbal extract comparing to model group (P-value= 0.001). The MWM results showed significant changes in path length and time for finding the hidden platform in all groups during test and the same change in the treated comparing to the control group in memory level. It could be concluded that the herbal extract may have significant effect on gene expression but not on behavioral level.
散发性阿尔茨海默病（AD）占病例总数的95%以上。AD的可能机制，如大脑中的炎症和氧化应激，促使研究人员探索多种有效的治疗方法，尤其是在疾病的早期阶段。IMOD是一种富含硒的犬蔷薇、普通百里香和 dioica 植物的草药提取物，具有抗炎、免疫调节和抗氧化应激的保护作用。在本研究中，选择了三个与AD相关的基因DAXX、NFκβ和VEGF作为候选基因，通过比较草药治疗组和对照组散发性AD大鼠模型海马体中的表达水平，利用qPCR研究提取物的神经保护作用。通过莫里斯水迷宫（MWM）试验评估对学习和记忆水平的治疗效果。基因表达结果表明，与模型组相比，草药提取物处理后大鼠海马体中Vegf显著上调（P值 = 0.001）。MWM结果显示，在测试期间，所有组寻找隐藏平台的路径长度和时间均有显著变化，与对照组相比，治疗组在记忆水平上也有相同变化。可以得出结论，草药提取物可能对基因表达有显著影响，但对行为水平没有影响。
