Department of Bioresources and Food Science, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Republic of Korea.
Department of Crop Science, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, Republic of Korea.
J Biochem Mol Toxicol. 2018 Jan;32(1). doi: 10.1002/jbt.22005. Epub 2017 Oct 26.
Xanthine oxidase (XO) found in all mammals and excess activity leads to urolithiasis. The cow milk XO was purified to 305-fold with a specific activity of 8.76 EU/mg and overall yield of 87% by using DEAE-Sepharose chromatography. The phenolics showed potent XO inhibitory effect with K , P1 (0.412), P2 (0.632), P3 (0.585), P4 (0.886), P5 (1.633), P6 (0.503), P7 (2.882), P8 (3.761), P9 (4.487), and P10 (5.841) μM. The phenolics P9 and P10 exhibited uncompetitive inhibition; the phenolics P1, P2, P3, P4, and P6 showed competitive inhibition, and other phenolic acids showed noncompetitive inhibition. The studied phenolic compounds showed potent antioxidant activity and expressed as EC , ranged from, DPPH (4.2-25.8 μg mL ), ABTS (10.2-42.5 mmol TE 100 g ), and FRAP (6.3-36.8 mol Fe (II) 100 g ). The results obtained from this study might be utilized for design of XO inhibitors and as antigout agent.
黄嘌呤氧化酶（XO）存在于所有哺乳动物中，其活性过高会导致尿结石。通过使用 DEAE-Sepharose 层析法，将牛乳 XO 纯化了 305 倍，比活为 8.76 EU/mg，总收率为 87%。酚类物质对 XO 具有很强的抑制作用，Ki、P1（0.412）、P2（0.632）、P3（0.585）、P4（0.886）、P5（1.633）、P6（0.503）、P7（2.882）、P8（3.761）、P9（4.487）和 P10（5.841）μM。酚类物质 P9 和 P10 表现出非竞争性抑制；酚类物质 P1、P2、P3、P4 和 P6 表现出竞争性抑制，而其他酚酸表现出非竞争性抑制。研究中的酚类化合物表现出很强的抗氧化活性，以 EC 表示，范围从 DPPH（4.2-25.8μg mL）、ABTS（10.2-42.5mmol TE 100g）和 FRAP（6.3-36.8mol Fe（II）100g）。本研究的结果可用于设计 XO 抑制剂和抗痛风药物。
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