Wu Yu, Peng Xiao-Qing, Jiang Xiao-Yan, Shi Mei-Qin, Yang Shui-Ying, Fu Ying-Jun, Peng Min, Cai Yan, Jiang Sheng-Shi, Xu Yan
Department of Pharmacy, Nantong Chinese Medicine Hospital, Nantong 226001, China.
Medical School of Nantong University, Nantong 226001, China.
Zhongguo Zhong Yao Za Zhi. 2017 Apr;42(8):1603-1608. doi: 10.19540/j.cnki.cjcmm.20170224.014.
Under the traditional processing theory "wine processing could promote the efficacy", Rhubarb after wine processing could treat the upper energizer diseases such as red swelling, and breath sores. Processing changes the medicinal properties of rhubarb, and thus results in different focuses in clinical application. In this study, a sensitive and specific method was developed for the determination of aloe-emodin, rhein and emodin in rats tissue. Rhubarb raw materials and its wine processed decoction were given to SD rats respectively by gavage administration, and then the contents of aloe-emodin, rhein and emodin in the tissues (heart, lung, brain, liver, kidney) were determined by HPLC-MS to explore the effect of wine processing on free anthraquinones in rat tissues. Experimental results showed that wine processing can significantly change the distribution of aloe emodin, rhein and emodin in rats in vivo, and the distribution of these components was increased in heart and lung tissues.There was no significant change of distribution in the liver and the kidney as compared with raw product group, and these three ingredients were not detected in the brain, indicating that aloe-emodin, rhein, emodin can not pass through the blood brain barrier.Therefore, wine processing had greater effect on distribution of free anthraquinones in rat tissues.This also verified the theory of traditional Chinese medicine, providing experimental basis for rhubarb processing mechanism.
在传统炮制理论“酒制能增效”的指导下，酒制大黄可治疗上部实热病症，如红肿、口疮等。炮制改变了大黄的药性，从而导致临床应用侧重点不同。本研究建立了一种灵敏、特异的方法用于测定大鼠组织中芦荟大黄素、大黄酸和大黄素的含量。将大黄原料及其酒制汤剂分别通过灌胃给予SD大鼠，然后采用高效液相色谱-质谱法测定组织（心、肺、脑、肝、肾）中芦荟大黄素、大黄酸和大黄素的含量，以探讨酒制对大鼠组织中游离蒽醌类成分的影响。实验结果表明，酒制可显著改变芦荟大黄素、大黄酸和大黄素在大鼠体内的分布，这些成分在心脏和肺组织中的分布增加。与生品组相比，肝脏和肾脏中的分布无显著变化，且在脑中未检测到这三种成分，表明芦荟大黄素、大黄酸、大黄素不能透过血脑屏障。因此，酒制对大鼠组织中游离蒽醌类成分的分布影响较大。这也验证了中医理论，为大黄炮制机制提供了实验依据。
