Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg , Robert-Koch-Straße 4, 35037 Marburg, Germany.
Fachbereich Chemie, Philipps-Universität Marburg , Hans Meerwein-Straße, 35032 Marburg, Germany.
Org Lett. 2017 Nov 3;19(21):5928-5931. doi: 10.1021/acs.orglett.7b02926.
Two prenyltransferases from Aspergillus ruber control the echinulin biosynthesis via exceptional sequential prenylations. EchPT1 catalyzes the first prenylation step, leading to preechinulin. The unique EchPT2 attaches, in a consecutive prenylation cascade, up to three dimethylallyl moieties to preechinulin and its dehydro forms neoechinulins A and B, resulting in the formation of at least 23 2- to 4-fold prenylated derivatives. Confirming these products in fungal extracts unravels the unprecedented catalytic relevance of EchPT2 for structural diversity.
两株红曲霉中的prenyltransferase 通过独特的顺序 prenylations 控制echinulin 的生物合成。EchPT1 催化第一个 prenylation 步骤，导致 preechinulin 的形成。独特的 EchPT2 在连续的 prenylation 级联中，将多达三个 dimethylallyl 部分连接到 preechinulin 及其脱氢形式的 neoechinulins A 和 B 上，导致至少 23 种 2 到 4 倍 prenylated 衍生物的形成。在真菌提取物中确认这些产物揭示了 EchPT2 对结构多样性的前所未有的催化相关性。
