Masiero Eva, Banik Dipanwita, Abson John, Greene Paul, Slater Adrian, Sgamma Tiziana
Biomolecular Technology Group, Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University, Leicester LE1 9BH, UK.
CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.
Plants (Basel). 2017 Oct 26;6(4):53. doi: 10.3390/plants6040053.
and species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between and taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five and samples from the UK National Plant Collections of and were analyzed. The 5' end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase () was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs.
[物种名称]在东亚作为传统药物有着悠久的使用历史。它们还在全球范围内被广泛用于观赏和景观美化目的。[物种名称]和[另一物种名称]类群之间的形态相似性使得这两个属的分类学存在问题，并导致了个体标本鉴定的混乱。分子方法结合传统方法可能是区分这两个属的有用工具。对来自英国国家植物标本馆的75份[物种名称]和[另一物种名称]样本进行了分析。利用核酮糖-1,5-二磷酸羧化酶/加氧酶（[酶名称]）大亚基基因的DNA条形码区域的5'端来区分这两个属。两个属之间的单核苷酸多态性（SNP）使得基于特异性PCR和高分辨率熔解曲线（HRM）分析开发出用于属水平鉴定的鉴别测试成为可能。该研究突出了将DNA条形码方法纳入植物鉴定方案的优势，并提供了可用于商业交易植物和草药质量保证的简单分析方法。
