Silva Mauricio, Urra Felipe, Ratto Marcelo
School of Veterinary Medicine, Universidad Católica de Temuco, Temuco, Chile; Núcleo de investigación en Producción Agroalimentaria, Universidad Católica de Temuco, Temuco, Chile.
School of Graduate Studies, Universidad Austral de Chile, Valdivia, Chile.
Theriogenology. 2018 Jan 15;106:164-169. doi: 10.1016/j.theriogenology.2017.10.027. Epub 2017 Oct 17.
The aim of this study was to evaluated changes in endometrial vascularization area (EVA) between the left and right uterine horn: a) during the ovarian follicular growth in intact llamas, and b) after exogenous estradiol administration of estradiol benzoate in ovariectomized (OVX) llamas. In experiment 1 follicle wave emergence was synchronized (n = 5 llamas) by follicle ablation (Day 0). Females were examined every other day from Day 1 to Day 27, using B mode ultrasonography to evaluate dominant follicle growth profile. Also, EVA was evaluated in each horn using Color-Power Doppler. Blood samples were taken every other day from Day 1 to Day 27 to measure estradiol (E2) plasma concentration by RIA. In experiment 2 OVX llamas (n = 4 llamas/group) were given a single im administration of: a) 1 mg of estradiol benzoate (EB) or b) 1 mL of saline. Females were subjected to ultrasound examinations every 48 h from Day -4 until treatment (Day 0), every 12 h from Day 0 to Day 4, and again every 48 h from Day 5 to Day 11. Evaluation of EVA in both uterine horns was performed as described for experiment 1. Blood sampling for the measurement of E2 was carried out at the same time points indicated for the ultrasound examinations. Serial data were analyzed by one way ANOVA for repeated measures using the MIXED Procedure in SAS. Also, Pearson's correlation was used to determine the relationship between variables. In intact llamas there was an effect of day on the dominant follicle growing profile (P < 0.01) and estradiol plasma concentration (P < 0.05). Dominant follicle diameter positively correlated (r = 0.4; P < 0.017) with estradiol plasma concentration. Also, EVA of right and left uterine horn did not differ (P = 0.89) during the evaluation period; however, it was affected by time (P < 0.05). In ovariectomized llamas estradiol concentration was significantly (P < 0.001) affected by treatment, time and their interaction. Accordingly, treatment with EB (P < 0.0001), time (P < 0.05) and their interaction (P < 0.01) affected EVA of both uterine horns; however, this variable did not differ between horns (P = 0.98). In conclusion, circulating concentrations of estradiol determined an increase in uterine vascularization, during the phase of follicular growth in intact llamas and after the exogenous administration of EB to ovariectomized females; however, no differential effect in endometrial vascularization area between right and left uterine horn was observed.
本研究的目的是评估左右子宫角之间子宫内膜血管化面积（EVA）的变化：a）在完整美洲驼卵巢卵泡生长期间，以及b）在给去卵巢（OVX）美洲驼注射苯甲酸雌二醇后。在实验1中，通过卵泡消融（第0天）使卵泡波同步出现（n = 5只美洲驼）。从第1天到第27天每隔一天对雌性进行检查，使用B型超声评估优势卵泡生长情况。此外，使用彩色能量多普勒评估每个子宫角的EVA。从第1天到第27天每隔一天采集血样，通过放射免疫分析法测量血浆雌二醇（E2）浓度。在实验2中，给去卵巢美洲驼（n = 4只/组）单次肌肉注射：a）1毫克苯甲酸雌二醇（EB）或b）1毫升生理盐水。从第-4天到治疗前（第0天）每48小时对雌性进行超声检查，从第0天到第4天每12小时检查一次，从第5天到第11天再次每48小时检查一次。按照实验1所述方法评估两个子宫角的EVA。在超声检查指定的相同时间点采集血样以测量E2。使用SAS中的MIXED过程通过单因素方差分析对重复测量的系列数据进行分析。此外，使用Pearson相关性分析确定变量之间的关系。在完整美洲驼中，天数对优势卵泡生长情况（P < 0.01）和血浆雌二醇浓度（P < 0.05）有影响。优势卵泡直径与血浆雌二醇浓度呈正相关（r = 0.4；P < 0.017）。此外，在评估期间左右子宫角的EVA没有差异（P = 0.89）；然而，它受时间影响（P < 0.05）。在去卵巢美洲驼中，雌二醇浓度受治疗、时间及其相互作用的显著影响（P < 0.001）。因此，EB治疗（P < 0.0001）、时间（P < 0.05）及其相互作用（P < 0.01）影响两个子宫角的EVA；然而，该变量在两个子宫角之间没有差异（P = 0.98）。总之，在完整美洲驼卵泡生长阶段以及给去卵巢雌性外源性注射EB后，循环雌二醇浓度导致子宫血管化增加；然而，未观察到左右子宫角之间子宫内膜血管化面积的差异效应。
