Gholami Hanieh, Jeddi Sajad, Zadeh-Vakili Azita, Farrokhfall Khadije, Rouhollah Fatemeh, Zarkesh Maryam, Ghanbari Mahboubeh, Ghasemi Asghar
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Physiol Biochem. 2017;43(6):2338-2352. doi: 10.1159/000484386. Epub 2017 Oct 27.
BACKGROUND/AIMS: Transient congenital hypothyroidism (TCH) could disturb carbohydrate metabolism in adulthood. Aging is associated with increased risk of type 2 diabetes. This study aims to address effects of TCH on mRNA expressions of glucose transporters (GLUTs) and glucokinase (GcK) in islets and insulin target tissues of aged offspring rats.
The TCH group received water containing 0.025% 6-propyl-2-thiouracil during gestation. Offspring from control and TCH groups (n=6 in each group) were followed until month 19. Gene expressions of GLUTs and GcK were measured at months 3 and 19.
Compared to controls, aged TCH rats had higher GLUT4 expression in heart (4.88 fold) and soleus (6.91 fold), while expression was lower in epididymal fat (12%). In TCH rats, GLUT2 and GcK expressions in islets were lower in young (12% and 10%, respectively) and higher in aged (10.85 and 8.42 fold, respectively) rats. In addition, liver GLUT2 and GcK expressions were higher in young (13.11 and 21.15 fold, respectively) and lower in aged rats (44% and 5%, respectively).
Thyroid hormone deficiency during fetal period impaired glucose sensing apparatus and changed glucose transporter expression in insulin-sensitive tissues of aged offspring rats. These changes may contribute to impaired carbohydrate metabolism.
背景/目的：短暂性先天性甲状腺功能减退症（TCH）可能会干扰成年期的碳水化合物代谢。衰老与2型糖尿病风险增加有关。本研究旨在探讨TCH对老年子代大鼠胰岛和胰岛素靶组织中葡萄糖转运蛋白（GLUTs）和葡萄糖激酶（GcK）mRNA表达的影响。
TCH组在妊娠期饮用含0.025% 6-丙基-2-硫脲嘧啶的水。对照组和TCH组的子代（每组n = 6）随访至19月龄。在3月龄和19月龄时测量GLUTs和GcK的基因表达。
与对照组相比，老年TCH大鼠心脏（4.88倍）和比目鱼肌（6.91倍）中GLUT4表达较高，而附睾脂肪中表达较低（12%）。在TCH大鼠中，胰岛中GLUT2和GcK的表达在幼年时较低（分别为12%和10%），在老年时较高（分别为10.85倍和8.42倍）。此外，肝脏中GLUT2和GcK的表达在幼年时较高（分别为13.11倍和21.15倍），在老年时较低（分别为44%和5%）。
胎儿期甲状腺激素缺乏损害了老年子代大鼠胰岛素敏感组织中的葡萄糖感应装置并改变了葡萄糖转运蛋白的表达。这些变化可能导致碳水化合物代谢受损。
