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触角足同源结构域的分离及序列特异性DNA结合

Isolation and sequence-specific DNA binding of the Antennapedia homeodomain.

作者信息

Müller M, Affolter M, Leupin W, Otting G, Wüthrich K, Gehring W J

机构信息

Biozentrum der Universität Basel, Switzerland.

出版信息

EMBO J. 1988 Dec 20;7(13):4299-304. doi: 10.1002/j.1460-2075.1988.tb03328.x.

DOI:10.1002/j.1460-2075.1988.tb03328.x
PMID:2907479
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC455145/
Abstract

The homeodomain encoded by the Antennapedia (Antp) gene of Drosophila was overproduced in a T7 expression vector in Escherichia coli. The corresponding polypeptide of 68 amino acids was purified to homogeneity. The homeodomain was analysed by ultracentrifugation and assayed for DNA binding. The secondary structure of the isolated homeodomain was determined by nuclear magnetic resonance spectroscopy. DNA-binding studies indicate that the isolated homeodomain binds to DNA in vitro. It selectively binds to the same sites as a longer Antp polypeptide and a full-length fushi tarazu (ftz) protein. Therefore, the homeodomain represents the DNA-binding domain of the homeotic proteins.

摘要

果蝇触角足（Antp）基因编码的同源异型结构域在大肠杆菌的T7表达载体中过量表达。由68个氨基酸组成的相应多肽被纯化至同质。通过超速离心分析该同源异型结构域，并检测其与DNA的结合能力。通过核磁共振光谱法确定分离出的同源异型结构域的二级结构。DNA结合研究表明，分离出的同源异型结构域在体外能与DNA结合。它与较长的Antp多肽和全长的分节基因（ftz）蛋白选择性地结合于相同位点。因此，同源异型结构域代表了同源异型蛋白的DNA结合结构域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/6eb9239e1d5a/emboj00150-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/e4acdfe5eb29/emboj00150-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/3359ba21541f/emboj00150-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/4c1ca343b80e/emboj00150-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/6eb9239e1d5a/emboj00150-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/e4acdfe5eb29/emboj00150-0272-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/3359ba21541f/emboj00150-0273-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/4c1ca343b80e/emboj00150-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ea8/455145/6eb9239e1d5a/emboj00150-0274-b.jpg

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本文引用的文献

1
Regulatory functions of the lambda repressor reside in the amino-terminal domain.lambda 阻遏物的调控功能位于氨基端结构域。
Nature. 1979 May 31;279(5712):396-400. doi: 10.1038/279396a0.
2
The operator-binding domain of lambda repressor: structure and DNA recognition.λ阻遏蛋白的操纵子结合结构域：结构与DNA识别
Nature. 1982 Jul 29;298(5873):443-7. doi: 10.1038/298443a0.
3
Many gene-regulatory proteins appear to have a similar alpha-helical fold that binds DNA and evolved from a common precursor.许多基因调控蛋白似乎具有相似的结合DNA的α螺旋结构，并且由一个共同的前体进化而来。
Angew Chem Int Ed Engl. 2020 Jan 20;59(4):1465-1468. doi: 10.1002/anie.201910960. Epub 2019 Dec 3.
4
Rapid and efficient purification of Drosophila homeodomain transcription factors for biophysical characterization.用于生物物理表征的果蝇同源域转录因子的快速高效纯化。
Protein Expr Purif. 2019 Jun;158:9-14. doi: 10.1016/j.pep.2019.02.001. Epub 2019 Feb 7.
5
Hox genes, evo-devo, and the case of the ftz gene.同源异型基因、演化发育生物学与ftz基因实例
Chromosoma. 2016 Jun;125(3):535-51. doi: 10.1007/s00412-015-0553-6. Epub 2015 Nov 23.
6
Entropic Enhancement of Protein-DNA Affinity by Oxygen-to-Sulfur Substitution in DNA Phosphate.通过DNA磷酸基团中氧到硫的取代实现蛋白质-DNA亲和力的熵增强
Biophys J. 2015 Sep 1;109(5):1026-37. doi: 10.1016/j.bpj.2015.07.032.
7
Walter J. Gehring (1939-2014).沃尔特·J·盖林（1939 - 2014）。
Dev Biol. 2014 Nov 1;395(1):1-3. doi: 10.1016/j.ydbio.2014.09.007.
8
NMR with (13)C, (15)N-doubly-labeled DNA: The shape Antennapedia homeodomain complex with a 14-mer DNA duplex.（13）C、（15）N 双标记 DNA 的 NMR：与 14 -mer DNA 双链的触角足同源域复合物的形状。
J Biomol NMR. 1998 Jul;12(1):25-37. doi: 10.1023/A:1008280117211.
9
Function and specificity of synthetic Hox transcription factors in vivo.体内合成 Hox 转录因子的功能和特异性。
Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4087-92. doi: 10.1073/pnas.0914595107. Epub 2010 Feb 10.
10
Cognate Site Identifier analysis reveals novel binding properties of the Sex Inducer homeodomain proteins of Cryptococcus neoformans.同源位点识别分析揭示了新型隐球菌性诱导同源结构域蛋白的新结合特性。
Mol Microbiol. 2009 Jun;72(6):1334-47. doi: 10.1111/j.1365-2958.2009.06719.x. Epub 2009 May 25.
J Mol Evol. 1983;19(2):109-14. doi: 10.1007/BF02300748.
4
Fly and frog homoeo domains show homologies with yeast mating type regulatory proteins.果蝇和青蛙的同源异型结构域与酵母交配型调节蛋白具有同源性。
Nature. 1984;310(5972):70-1. doi: 10.1038/310070a0.
5
Spatial arrangement of the three alpha helices in the solution conformation of E. coli lac repressor DNA-binding domain.大肠杆菌乳糖阻遏蛋白DNA结合结构域溶液构象中三个α螺旋的空间排列。
FEBS Lett. 1984 Sep 3;174(2):243-7. doi: 10.1016/0014-5793(84)81166-7.
6
Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila.控制发育的基因间的结构关系：果蝇触角足、超双胸和腹沟基因座之间的序列同源性
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4115-9. doi: 10.1073/pnas.81.13.4115.
7
Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.果蝇体节基因的序列：与DNA结合蛋白的蛋白质结构同源性
Nature. 1984;310(5972):25-31. doi: 10.1038/310025a0.
8
Cloning of an X. laevis gene expressed during early embryogenesis coding for a peptide region homologous to Drosophila homeotic genes.克隆在早期胚胎发生过程中表达的非洲爪蟾基因，该基因编码一个与果蝇同源异型基因同源的肽段区域。
Cell. 1984 Jun;37(2):409-14. doi: 10.1016/0092-8674(84)90371-4.
9
A homologous protein-coding sequence in Drosophila homeotic genes and its conservation in other metazoans.果蝇同源异型基因中的一个同源蛋白质编码序列及其在其他后生动物中的保守性。
Cell. 1984 Jun;37(2):403-8. doi: 10.1016/0092-8674(84)90370-2.
10
A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes.果蝇触角足复合体和双胸复合体同源异型基因中的一个保守DNA序列。
Nature. 1984;308(5958):428-33. doi: 10.1038/308428a0.