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通过核磁共振确定触角足同源结构域的二级结构以及螺旋-转角-螺旋基序的证据。

Secondary structure determination for the Antennapedia homeodomain by nuclear magnetic resonance and evidence for a helix-turn-helix motif.

作者信息

Otting G, Qian Y Q, Müller M, Affolter M, Gehring W, Wüthrich K

机构信息

Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule-Hönggerberg, Zürich, Switzerland.

出版信息

EMBO J. 1988 Dec 20;7(13):4305-9. doi: 10.1002/j.1460-2075.1988.tb03329.x.

DOI:10.1002/j.1460-2075.1988.tb03329.x
PMID:2907480
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC455146/
Abstract

The homeodomain encoded by the Antennapedia (Antp) gene of Drosophila was studied in aqueous solution by nuclear magnetic resonance (NMR). Sequence-specific resonance assignments have been obtained for the complete polypeptide chain of 68 amino acid residues. The secondary structure determined from nuclear Overhauser effects (NOE) and information about slowly exchanging amide protons includes three helical segments consisting of the residues 10-21, 28-38 and 42-52, respectively. Combination of the presently available NMR data with computer modeling provided preliminary evidence for the presence of a helix-turn-helix motif in the homeodomain. Near the turn, this supersecondary structure appears to be very similar to the DNA binding site in the 434 and P22 c2 repressors, but both helices in the homeodomain include 2-3 additional residues when compared with these prokaryotic DNA-binding proteins.

摘要

利用核磁共振（NMR）技术，在水溶液中对果蝇触角足（Antp）基因编码的同源结构域进行了研究。已获得了由68个氨基酸残基组成的完整多肽链的序列特异性共振归属。根据核Overhauser效应（NOE）以及有关缓慢交换酰胺质子的信息确定的二级结构包括三个螺旋片段，分别由10 - 21、28 - 38和42 - 52位残基组成。将目前可得的NMR数据与计算机建模相结合，为同源结构域中存在螺旋-转角-螺旋基序提供了初步证据。在转角附近，这种超二级结构似乎与434和P22 c2阻遏物中的DNA结合位点非常相似，但与这些原核DNA结合蛋白相比，同源结构域中的两个螺旋都包含2 - 3个额外的残基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c91b/455146/ed2e8eae07b2/emboj00150-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c91b/455146/ed2e8eae07b2/emboj00150-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c91b/455146/ed2e8eae07b2/emboj00150-0279-a.jpg

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