Che Zhijun, Liu Hailun, Yi Fanglei, Cheng Hao, Yang Yuming, Wang Li, Du Jingyi, Zhang Peipei, Wang Jiao, Yu Deyue
National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, China.
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
Front Plant Sci. 2017 Oct 11;8:1771. doi: 10.3389/fpls.2017.01771. eCollection 2017.
Soybean mosaic virus (SMV) is a member of genus that causes severe yield loss and destroys seed quality in soybean [ (L.) Merr.]. It is important to explore new resistance sources and discover new resistance loci to SMV, which will provide insights to improve breeding strategies for SMV resistance. Here, a genome-wide association study was conducted to accelerate molecular breeding for the improvement of resistance to SMV in soybean. A population of 165 soybean mutants derived from two soybean parents was used in this study. There were 104 SNPs identified significantly associated with resistance to SC7, some of which were located within previous reported quantitative trait loci. Three putative genes on chromosome 1, 9, and 12 were homologous to , and , which were involved in defense response to insect and disease in . Moreover, the expression levels of these three genes changed in resistance and susceptible soybean accessions after SMV infection. These three putative genes may involve in the resistance to SC7 and be worthy to further research. Collectively, markers significantly associated with resistance to SC7 will be helpful in molecular marker-assisted selection for breeding resistant soybean accessions to SMV, and the candidate genes identified would advance the functional study of resistance to SMV in soybean.
大豆花叶病毒（SMV）是一种病毒属成员，可导致大豆[（L.）Merr.]严重减产并破坏种子质量。探索新的抗源并发现新的SMV抗性位点很重要，这将为改进SMV抗性育种策略提供思路。在此，进行了一项全基因组关联研究，以加速大豆抗SMV的分子育种。本研究使用了由两个大豆亲本衍生的165个大豆突变体群体。鉴定出104个与SC7抗性显著相关的单核苷酸多态性（SNP），其中一些位于先前报道的数量性状位点内。位于第1、9和12号染色体上的三个推定基因与[具体基因名称未给出]同源，这些基因参与了[具体物种未给出]对昆虫和疾病的防御反应。此外，在SMV感染后，这三个基因在抗性和易感大豆材料中的表达水平发生了变化。这三个推定基因可能参与对SC7的抗性，值得进一步研究。总体而言，与SC7抗性显著相关的标记将有助于分子标记辅助选择培育抗SMV的大豆材料，并且鉴定出的候选基因将推动大豆抗SMV功能研究的进展。
