Kim Jung Min, Lee Jeong Woo, Seo Ji Su, Ha Bo-Keun, Kwon Soon-Jae
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea.
Department of Applied Plant Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Plants (Basel). 2024 Feb 21;13(5):584. doi: 10.3390/plants13050584.
Soybean [ (L.) Merr.] isoflavones, which are secondary metabolites with various functions, are included in food, cosmetics, and medicine. However, the molecular mechanisms regulating the glycosylation and malonylation of isoflavone glycoconjugates remain unclear. In this study, we conducted an RNA-seq analysis to compare soybean genotypes with different isoflavone contents, including Danbaek and Hwanggeum (low-isoflavone cultivars) as well as DB-088 (high-isoflavone mutant). The transcriptome analysis yielded over 278 million clean reads, representing 39,156 transcripts. The analysis of differentially expressed genes (DEGs) detected 2654 up-regulated and 1805 down-regulated genes between the low- and high-isoflavone genotypes. The putative functions of these 4459 DEGs were annotated on the basis of GO and KEGG pathway enrichment analyses. These DEGs were further analyzed to compare the expression patterns of the genes involved in the biosynthesis of secondary metabolites and the genes encoding transcription factors. The examination of the relative expression levels of 70 isoflavone biosynthetic genes revealed the , , , and expression levels were significantly up/down-regulated depending on the genotype and seed developmental stage. These expression patterns were confirmed by quantitative real-time PCR. Moreover, a gene co-expression analysis detected potential protein-protein interactions, suggestive of common functions. The study findings provide valuable insights into the structural genes responsible for isoflavone biosynthesis and accumulation in soybean seeds.
大豆[ (L.) Merr.]异黄酮是具有多种功能的次生代谢产物，存在于食品、化妆品和药品中。然而，调控异黄酮糖缀合物糖基化和丙二酰化的分子机制仍不清楚。在本研究中，我们进行了RNA测序分析，以比较异黄酮含量不同的大豆基因型，包括丹白和黄金（低异黄酮品种）以及DB - 088（高异黄酮突变体）。转录组分析产生了超过2.78亿条clean reads，代表39,156个转录本。差异表达基因（DEG）分析检测到低异黄酮和高异黄酮基因型之间有2654个上调基因和1805个下调基因。基于GO和KEGG通路富集分析对这4459个DEG的推定功能进行了注释。对这些DEG进一步分析，以比较参与次生代谢物生物合成的基因和编码转录因子的基因的表达模式。对70个异黄酮生物合成基因相对表达水平的检测表明， 、 、 和 的表达水平根据基因型和种子发育阶段显著上调/下调。这些表达模式通过定量实时PCR得到证实。此外，基因共表达分析检测到潜在的蛋白质-蛋白质相互作用，提示其具有共同功能。该研究结果为大豆种子中负责异黄酮生物合成和积累的结构基因提供了有价值的见解。
