School of Kinesiology, Auburn University, Auburn, AL, USA.
Department of Cell Biology and Physiology, Edward Via College of Osteopathic Medicine, Auburn Campus, 910 S. Donahue Drive, Auburn, AL, 36832, USA.
Eur J Appl Physiol. 2017 Dec;117(12):2587-2600. doi: 10.1007/s00421-017-3746-2. Epub 2017 Oct 26.
To compare the effects of external pneumatic compression (EPC) and sham when used concurrently with high intensity interval training (HIIT) on performance-related outcomes and recovery-related molecular measures.
Eighteen recreationally endurance-trained male participants (age: 21.6 ± 2.4 years, BMI: 25.7 ± 0.5 kg/m, VO: 51.3 ± 0.9 mL/kg/min) were randomized to balanced sham and EPC treatment groups. Three consecutive days of HIIT followed by EPC/sham treatment (Days 2-4) and 3 consecutive days of recovery (Days 5-7) with EPC/sham only on Days 5-6 were employed. Venipuncture, flexibility and pressure-to-pain threshold (PPT) measurements were made throughout. Vastus lateralis muscle was biopsied at PRE (i.e., Day 1), 1-h post-EPC/sham treatment on Day 2 (POST1), and 24-h post-EPC/sham treatment on Day 7 (POST2). 6-km run time trial performance was tested at PRE and POST2.
No group × time interaction was observed for flexibility, PPT, or serum measures of creatine kinase (CK), hsCRP, and 8-isoprostane. However, there was a main effect of time for serum CK (p = 0.005). Change from PRE in 6-km run times at POST2 were not significantly different between groups. Significant between-groups differences existed for change from PRE in atrogin-1 mRNA (p = 0.018) at the POST1 time point (EPC: - 19.7 ± 8.1%, sham: + 7.7 ± 5.9%) and atrogin-1 protein concentration (p = 0.013) at the POST2 time point (EPC: - 31.8 ± 7.5%, sham: + 96.0 ± 34.7%). In addition, change from PRE in poly-Ub proteins was significantly different between groups at both the POST1 (EPC: - 26.0 ± 10.3%, sham: + 34.8 ± 28.5%; p = 0.046) and POST2 (EPC: - 33.7 ± 17.2%, sham: + 21.4 ± 14.9%; p = 0.037) time points.
EPC when used concurrently with HIIT and in subsequent recovery days reduces skeletal muscle markers of proteolysis.
比较外部气动压缩（EPC）与假对照在高强度间歇训练（HIIT）同时应用时对相关表现和恢复相关分子指标的影响。
18 名有经验的耐力训练男性参与者（年龄：21.6±2.4 岁，BMI：25.7±0.5kg/m，VO₂：51.3±0.9mL/kg/min）被随机分配到平衡的假对照和 EPC 治疗组。连续 3 天进行 HIIT，然后在第 2-4 天进行 EPC/假对照治疗，仅在第 5-6 天进行 EPC/假对照治疗，进行 3 天恢复。在整个过程中进行静脉穿刺、柔韧性和压力-疼痛阈值（PPT）测量。在 PRE（即第 1 天）、第 2 天 EPC/假对照治疗后 1 小时（POST1）和第 7 天 EPC/假对照治疗后 24 小时（POST2）对股外侧肌进行活检。在 PRE 和 POST2 进行 6km 跑步时间试验。
在柔韧性、PPT 或血清肌酸激酶（CK）、hsCRP 和 8-异前列腺素水平方面，未观察到组×时间的交互作用。然而，血清 CK 存在时间的主效应（p=0.005）。POST2 时 6km 跑步时间从 PRE 的变化在组间没有显著差异。在 POST1 时间点（EPC：-19.7±8.1%，假对照：+7.7±5.9%）和 POST2 时间点（EPC：-31.8±7.5%，假对照：+96.0±34.7%），atrogin-1 mRNA 的 PRE 变化之间存在显著的组间差异。此外，在 POST1（EPC：-26.0±10.3%，假对照：+34.8±28.5%；p=0.046）和 POST2（EPC：-33.7±17.2%，假对照：+21.4±14.9%；p=0.037）时间点，多聚-Ub 蛋白的 PRE 变化在组间也有显著差异。
EPC 与 HIIT 同时应用，并在随后的恢复日中使用，可减少骨骼肌蛋白水解标志物。
