Takeuchi T, Matsuda H, Okuzawa E, Nozaki T, Kobayashi S, Tanaka H
Department of Parasitology, School of Medicine, Keio University, Tokyo, Japan.
Jpn J Exp Med. 1988 Oct;58(5):229-32.
Techniques of a micro enzyme-linked immunosorbent assay (ELISA) used for the serodiagnosis of schistosomiasis were applied to amebic infection. Test sera were divided primary on the basis of serologic diagnosis and stool examination as follows; (I) gel diffusion precipitin test (GDP) positive and stool examination positive: 9 specimens, (II) GDP positive and stool examination negative: 29 specimens, (III) GDP negative and stool examination positive: 32 specimens. Virtually all of the individuals belonging to (III) were asymptomatic, while more than 75% of (I) and (II) were symptomatic. The upper limit of 99% critical range was calculated from the data of 70 serum specimens from healthy adult Japanese and was employed as the cut-off value. All of the specimens of (I) and (II) were judged positive by ELISA, generally with a much higher absorbance than the cut-off value; whereas, approximately 80% of (III) were judged positive. The average absorbance of (III) was lower than that of (I) and (II). These findings suggest that the ELISA is well in accord with GDP qualitatively as far as GDP-positive individuals are concerned, and that even asymptomatic cyst carriers with negative serology by GDP may often be producing anti-amebic antibodies, although the titers are low.
用于血吸虫病血清诊断的微量酶联免疫吸附测定（ELISA）技术被应用于阿米巴感染。检测血清首先根据血清学诊断和粪便检查进行如下分类：（I）凝胶扩散沉淀试验（GDP）阳性且粪便检查阳性：9份标本；（II）GDP阳性且粪便检查阴性：29份标本；（III）GDP阴性且粪便检查阳性：32份标本。实际上，属于（III）组的所有个体均无症状，而（I）组和（II）组中超过75%的个体有症状。根据70名健康成年日本人的血清标本数据计算出99%临界范围的上限，并将其用作临界值。（I）组和（II）组的所有标本经ELISA判断均为阳性，其吸光度通常远高于临界值；而（III）组中约80%的标本被判断为阳性。（III）组的平均吸光度低于（I）组和（II）组。这些发现表明，就GDP阳性个体而言，ELISA在定性上与GDP非常一致，并且即使是GDP血清学阴性的无症状囊肿携带者也可能经常产生抗阿米巴抗体，尽管其滴度较低。
