孟加拉国用于诊断肝阿米巴病的间接荧光抗体试验和酶联免疫吸附测定的评估。

Evaluation of indirect fluorescent antibody test and enzyme-linked immunosorbent assay for diagnosis of hepatic amebiasis in Bangladesh.

作者信息

Shamsuzzaman S M, Haque R, Hasin S K, Hashiguchi Y

机构信息

Department of Parasitology, Kochi Medical School, Japan.

出版信息

J Parasitol. 2000 Jun;86(3):611-5. doi: 10.1645/0022-3395(2000)086[0611:EOIFAT]2.0.CO;2.

DOI:10.1645/0022-3395(2000)086[0611:EOIFAT]2.0.CO;2

PMID:10864262

Abstract

Serum samples of 31 amebic liver abscess (ALA) patients, 8 amebic hepatitis (AH) patients, and 60 controls were tested for anti-amebic IgG by enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody tests (IFAT). Sera of 29 (93.6%) ALA and 6 (75%) AH patients and 2 (3.3%) control subjects were positive by IFAT. Anti-amebic antibody titer above the cutoff point (= 0.168; x + 2 SD of control sera) was observed in sera of 27 (87%) ALA, 4 (50%) AH, and 1 (1.7%) control by ELISA. All the 8 pus samples were positive for anti-amebic antibodies by IFAT and ELISA. Sensitivity of ELISA was 87% for ALA, with a positive predictive value of 0.96, and 50% for AH cases, with a positive predictive value of 0.80. The sensitivity of IFAT was 93.6% for ALA, with a positive predictive value of 0.94, and 75% for AH, with a positive predictive value of 0.75. When pus samples were tested, the sensitivity was 100% for both tests. The specificity was 98.3% for ELISA and 96.7% for IFAT. Although not significant, IFAT was found more sensitive than ELISA (P>0.05).

摘要

采用酶联免疫吸附测定（ELISA）和间接荧光抗体试验（IFAT），对31例阿米巴肝脓肿（ALA）患者、8例阿米巴肝炎（AH）患者的血清样本以及60例对照者的血清样本进行抗阿米巴IgG检测。IFAT检测显示，29例（93.6%）ALA患者、6例（75%）AH患者以及2例（3.3%）对照者的血清呈阳性。ELISA检测发现，27例（87%）ALA患者、4例（50%）AH患者以及1例（1.7%）对照者的血清抗阿米巴抗体滴度高于临界值（= 0.168；对照血清的x + 2标准差）。所有8份脓液样本经IFAT和ELISA检测，抗阿米巴抗体均呈阳性。ELISA对ALA的敏感性为87%，阳性预测值为0.96；对AH病例的敏感性为50%，阳性预测值为0.80。IFAT对ALA的敏感性为93.6%，阳性预测值为0.94；对AH的敏感性为75%，阳性预测值为0.75。检测脓液样本时，两种检测方法的敏感性均为100%。ELISA的特异性为98.3%，IFAT的特异性为96.7%。尽管差异无统计学意义，但发现IFAT比ELISA更敏感（P>0.05）。