Dong Bingqi, Calik Ali, Blue Candice E C, Dalloul Rami A
Department of Poultry Science, University of Georgia, Athens, GA 30602, United States.
Department of Poultry Science, University of Georgia, Athens, GA 30602, United States; Department of Animal Nutrition & Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, Ankara 06110, Türkiye.
Poult Sci. 2024 Dec;103(12):104420. doi: 10.1016/j.psj.2024.104420. Epub 2024 Oct 13.
Necrotic enteritis (NE), an enteric disease caused by Clostridium perfringens, results in damage to the intestinal epithelial lining disrupting its function, nutrient absorption, and utilization. This study evaluated the effects of in ovo and post-hatch applications of a Saccharomyces cerevisiae-based postbiotic on performance and nutrient transporter genes of broilers during a NE challenge. At embryonic d 18, Ross 708 fertile eggs were injected with 0.2 mL of either water or postbiotic. A total of 288 male hatchlings were assigned to one of the following treatment groups: 1) NC (in ovo water injection, no challenge); 2) PIW (postbiotic in ovo and in drinking water, no challenge); 3) NC+ (NC with NE challenge); and 4) PIW+ (PIW with NE challenge). On d 14, all birds in the NE-challenged groups were orally gavaged with 3,000 Eimeria maxima sporulated oocysts followed by two doses of ∼1×10 CFU/mL/bird of C. perfringens on d 19 and d 20. Hatchability, weekly performance, intestinal lesion scores, and mRNA abundance of nutrient transporters in the jejunum and ileum were assessed. Data were analyzed by 2-way ANOVA in JMP and significance between treatments identified by LSD test (P ≤ 0.05). A significant postbiotic treatment and NE challenge interaction was observed in performance during d 21-28 with a greater ADG in PIW compared to NC and PIW+. Lesion scores in the jejunum and ileum were significantly reduced in PIW+ compared to NC+. On d 7, mRNA abundance of SGLT1 was significantly greater in PIW compared to the NC group. On d 14, birds in PIW had greater levels of GLUT2 and EAAT3 than NC group. No significant interaction effects were observed on d 21. PIW+ had significantly greater EAAT3 mRNA levels compared to PIW in jejunum and PIW and NC+ in ileum on d 28. In conclusion, in ovo and water supplementation of this postbiotic presents a potential to improve the performance, ameliorate pathology detriments associated with NE, and positively regulate the mRNA levels of key nutrient transporters during NE challenge.
坏死性肠炎（NE）是由产气荚膜梭菌引起的一种肠道疾病，会导致肠道上皮内层受损，破坏其功能、营养物质吸收和利用。本研究评估了基于酿酒酵母的后生元在胚胎期和出壳后应用对肉鸡在坏死性肠炎攻毒期间的生产性能和营养转运蛋白基因的影响。在胚胎第18天，给罗斯708种蛋注射0.2 mL水或后生元。总共288只雄性雏鸡被分配到以下处理组之一：1）NC（胚胎期注射水，未攻毒）；2）PIW（胚胎期和饮水中添加后生元，未攻毒）；3）NC+（NC组进行坏死性肠炎攻毒）；4）PIW+（PIW组进行坏死性肠炎攻毒）。在第14天，给所有坏死性肠炎攻毒组的鸡口服3000个巨型艾美耳球虫孢子化卵囊，随后在第19天和第20天给每只鸡口服两剂约1×10⁸ CFU/mL的产气荚膜梭菌。评估孵化率、每周生产性能、肠道病变评分以及空肠和回肠中营养转运蛋白的mRNA丰度。数据在JMP中通过双向方差分析进行分析，并通过LSD检验确定处理之间的显著性（P≤0.05）。在第21 - 28天的生产性能方面观察到后生元处理与坏死性肠炎攻毒之间存在显著的交互作用，与NC组和PIW+组相比，PIW组的平均日增重更高。与NC+组相比，PIW+组空肠和回肠的病变评分显著降低。在第7天，与NC组相比，PIW组中SGLT1的mRNA丰度显著更高。在第14天，PIW组的鸡的GLUT2和EAAT3水平高于NC组。在第21天未观察到显著的交互作用。在第28天，PIW+组空肠中EAAT3的mRNA水平显著高于PIW组，回肠中EAAT3的mRNA水平显著高于PIW组和NC+组。总之，胚胎期和饮水中添加这种后生元具有提高生产性能、改善与坏死性肠炎相关的病理损害以及在坏死性肠炎攻毒期间正向调节关键营养转运蛋白mRNA水平的潜力。
