Maastricht University, Aachen-Maastricht Institute for Biobased Materials (AMIBM), Brightlands Chemelot Campus, Urmonderbaan 22, 6167 RD, Geleen, The Netherlands.
Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands.
Chembiochem. 2018 Feb 16;19(4):354-360. doi: 10.1002/cbic.201700427. Epub 2017 Nov 29.
Baeyer-Villiger monooxygenases (BVMOs) are biocatalysts that are able to convert cyclic ketones into lactones by the insertion of oxygen. The aim of this study was to explore the substrate scope of several BVMOs with (biobased) cyclic ketones as precursors for the synthesis of branched polyesters. The product structure and the degree of conversion of several biotransformations were determined after conversions by using self-sufficient BVMOs. Full regioselectivity towards the normal lactones of jasmatone and menthone was observed, whereas the oxidation of other substrates such as α,β-thujone and 3,3,5-trimethylcyclohexanone resulted in mixtures of regioisomers. This exploration of the substrate scope of both established and newly discovered BVMOs towards biobased ketones contributes to the development of branched polyesters from renewable resources.
Baeyer-Villiger 单加氧酶（BVMOs）是一类能够通过插入氧原子将环状酮转化为内酯的生物催化剂。本研究旨在探索几种 BVMO 对（生物基）环状酮作为合成支化聚酯前体的底物范围。通过使用自给自足的 BVMO 进行转化后，确定了几种生物转化的产物结构和转化率。观察到茉莉酮和薄荷酮的正常内酯具有完全的区域选择性，而其他底物如α,β-蒎烯和 3,3,5-三甲基环己酮的氧化则导致区域异构体的混合物。对已建立和新发现的 BVMO 对生物基酮的底物范围的探索有助于从可再生资源中开发支化聚酯。
