College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Prog Biophys Mol Biol. 2019 Nov;148:39-46. doi: 10.1016/j.pbiomolbio.2017.10.003. Epub 2017 Oct 25.
Hydrophilic material 2-methoxyethylacrylate (MEA) was grafted onto polyurethane (PU) membrane via Michael addition reaction. Fourier transform infrared spectroscope (FTIR) and X-ray photoelectron spectroscopy (XPS) characterizations of the modified membrane proved that MEA was successfully grafted onto PU membrane surface. The water contact angle of the modified PU membrane decreased from 86° to 48° compared with unmodified PU membrane, which means that the hydrophilicity of the modified membrane was greatly improved. A series of blood compatibility tests including bovine serum protein adsorption, platelet adhesion, hemolysis assay, plasma recalacification time, prothrombin time (PT), partial thromboplastin time (APTT) and thrombin time (TT) were carried out on PU membrane and the modified PU membrane with highest grafted density of MEA. The combined results indicate that MEA plays an important role in improving the blood compatibility of PU membrane.
通过迈克尔加成反应，将亲水性材料 2-甲氧基乙基丙烯酸酯（MEA）接枝到聚氨酯（PU）膜上。改性膜的傅里叶变换红外光谱（FTIR）和 X 射线光电子能谱（XPS）表征证明 MEA 成功接枝到 PU 膜表面。与未改性的 PU 膜相比，改性 PU 膜的水接触角从 86°降低到 48°，这意味着改性膜的亲水性得到了极大的提高。在具有最高 MEA 接枝密度的 PU 膜和改性 PU 膜上进行了一系列血液相容性测试，包括牛血清蛋白吸附、血小板黏附、溶血试验、血浆再钙化时间、凝血酶原时间（PT）、部分凝血活酶时间（APTT）和凝血酶时间（TT）。综合结果表明，MEA 在提高 PU 膜的血液相容性方面起着重要作用。
