Han D K, Jeong S Y, Kim Y H, Min B G, Cho H I
Polymer Chemistry Laboratory, Korea Institute of Science and Technology, Cheongryang, Seoul.
J Biomed Mater Res. 1991 May;25(5):561-75. doi: 10.1002/jbm.820250502.
In order to investigate the interaction between various sulfonated polyurethanes (PUs) and blood, a commercial PU surface was chemically modified by poly(ethylene oxide) (PEO), dodecanediol(DDO), and propane sultone to give hydrophilic, hydrophobic, and negative sulfonated surfaces, respectively. The blood compatibility of modified PUs was evaluated by an in vitro platelet adhesion test, activated partial thromboplastin time (APTT), and prothrombin time (PT) measurements as well as an ex vivo rabbit A-A shunt method. In the platelet adhesion test, the hydrophilic PEO grafted PUs showed less platelet adhesion than untreated PU and hydrophobic DDO grafted PU. Sulfonated PU-PEO exhibited a lower degree of adhesion and shape change of platelet. The APTT and PT, especially APTT, of the sulfonated PUs were extended, whereas those of PU-PEO and PU-DDO did not show any significant change compared with untreated PU. Meanwhile, in the ex vivo experiment, hydrophilic PEO grafted PUs showed longer occlusion times than untreated PU or hydrophobic DDO grafted PU. In addition, the incorporation of SO3 groups at the end of PU-DDO and PU-PEO, particularly PU-PEO-SO3, exhibited an enormous prolongation in occlusion time, indicating a synergistic effect of the hydrophilic PEO and the negative SO3 groups on thromboresistance. These occlusion times corresponded well to in vitro evaluation results: the less adhesion and shape change of platelet and the longer APTT and PT, the more extended the ex vivo occlusion time.
为了研究各种磺化聚氨酯（PU）与血液之间的相互作用，通过聚环氧乙烷（PEO）、十二烷二醇（DDO）和丙烷磺内酯对商用PU表面进行化学改性，分别得到亲水性、疏水性和负磺化表面。通过体外血小板黏附试验、活化部分凝血活酶时间（APTT）和凝血酶原时间（PT）测量以及体外兔A-A分流法评估改性PU的血液相容性。在血小板黏附试验中，亲水性PEO接枝的PU比未处理的PU和疏水性DDO接枝的PU表现出更少的血小板黏附。磺化PU-PEO表现出较低程度的血小板黏附和形状变化。磺化PU的APTT和PT，尤其是APTT延长，而PU-PEO和PU-DDO与未处理的PU相比没有显示出任何显著变化。同时，在体外实验中，亲水性PEO接枝的PU比未处理的PU或疏水性DDO接枝的PU表现出更长的闭塞时间。此外，在PU-DDO和PU-PEO末端引入SO3基团，特别是PU-PEO-SO3，表现出闭塞时间的极大延长，表明亲水性PEO和负性SO3基团对血栓抗性具有协同作用。这些闭塞时间与体外评估结果非常吻合：血小板黏附和形状变化越少，APTT和PT越长，体外闭塞时间就越长。
