Institute for Biological Control, Federal Research Centre for Cultivated Plants, Julius Kühn Institute Heinrichtstraße 243, 64287 Darmstadt, Germany.
Institute for Biological Control, Federal Research Centre for Cultivated Plants, Julius Kühn Institute Heinrichtstraße 243, 64287 Darmstadt, Germany; Queen's University, Kingston K7L 3N6 Ontario, Canada.
J Invertebr Pathol. 2018 Jan;151:7-13. doi: 10.1016/j.jip.2017.10.005. Epub 2017 Oct 25.
Both Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV) belong to a cluster of four baculoviruses that are infective for different Agrotis species. Belonging further to different baculovirus genera, namely Alphabaculovirus and Betabaculovirus, respectively, AgseNPV-B and AgseGV are candidates to investigate virus interactions in co-infections. However, for the investigation of virus interactions on a cellular level, permissive insect cell-lines are needed. The cell line AiE1611T deriving from Agrotisipsilon eggs has been shown to be permissive for several Alphabaculovirus isolates. In this study, virus replication was followed based on microscopic analysis of infected and transfected cells, as well as on a molecular level by PCR of DNA and cDNA of selected baculovirus transcripts. While the permissivity was not verified for AgseGV, AgseNPV-B produced occlusion bodies in both infection with hemolymph of infected larvae and Lipofectamin transfection with AgseNPV-B genomic DNA. In addition to the possibility to investigate virus interaction of AgseNPV-B with other alphabaculoviruses, the permissivity of AiE1611T for AgseNPV-B further offers the possibility a biological selection to separate AgseNPV-B from AgseGV.
甜菜夜蛾核多角体病毒 B（AgseNPV-B）和甜菜夜蛾颗粒体病毒（AgseGV）都属于一组可感染不同夜蛾属物种的杆状病毒。AgseNPV-B 属于 Alphabaculovirus，而 AgseGV 属于 Betabaculovirus，分别属于不同的杆状病毒属，是研究共感染中病毒相互作用的候选病毒。然而，为了在细胞水平上研究病毒相互作用，需要有可允许的昆虫细胞系。源自 Agrotis ipsilon 卵的细胞系 AiE1611T 已被证明可允许几种 Alphabaculovirus 分离株感染。在本研究中，通过感染和转染细胞的显微镜分析以及选择的杆状病毒转录物的 DNA 和 cDNA 的 PCR 分子水平，跟踪了病毒的复制。虽然 AgseGV 的允许性未得到验证，但在感染幼虫血淋巴和用 AgseNPV-B 基因组 DNA 进行 Lipofectamin 转染时，AgseNPV-B 产生了包含体。除了研究 AgseNPV-B 与其他α杆状病毒的相互作用外，AiE1611T 对 AgseNPV-B 的允许性还提供了从 AgseGV 中分离 AgseNPV-B 的生物选择的可能性。
