Gueli Alletti Gianpiero, Eigenbrod Marina, Carstens Eric B, Kleespies Regina G, Jehle Johannes A
Institute for Biological Control, Federal Research Centre for Cultivated Plants, Julius Kühn Institute, Heinrichstraβe 243, 64287 Darmstadt, Germany.
Institute for Biological Control, Federal Research Centre for Cultivated Plants, Julius Kühn Institute, Heinrichstraβe 243, 64287 Darmstadt, Germany; Technische Universität Darmstadt, Karolinenplatz 5, 64289 Darmstadt, Germany.
J Invertebr Pathol. 2017 Jun;146:58-68. doi: 10.1016/j.jip.2017.04.008. Epub 2017 Apr 22.
The European isolate Agrotis segetum granulovirus DA (AgseGV-DA) is a slow killing, type I granulovirus due to low dose-mortality responses within seven days post infection and a tissue tropism of infection restricted solely to the fat body of infected Agrotis segetum host larvae. The genome of AgseGV-DA was completely sequenced and compared to the whole genome sequences of the Chinese isolates AgseGV-XJ and AgseGV-L1. All three isolates share highly conserved genomes. The AgseGV-DA genome is 131,557bp in length and encodes for 149 putative open reading frames, including 37 baculovirus core genes and the per os infectivity factor ac110. Comprehensive investigations of repeat regions identified one putative non-hr like origin of replication in AgseGV-DA. Phylogenetic analysis based on concatenated amino acid alignments of 37 baculovirus core genes as well as pairwise distances based on the nucleotide alignments of partial granulin, lef-8 and lef-9 sequences with deposited betabaculoviruses confirmed AgseGV-DA, AgseGV-XJ and AgseGV-L1 as representative isolates of the same Betabaculovirus species. AgseGV encodes for a distinct putative enhancin, distantly related to enhancins from other granuloviruses.
欧洲分离株黄地老虎颗粒体病毒DA（AgseGV - DA）是一种致死速度缓慢的I型颗粒体病毒，这是由于在感染后七天内低剂量死亡率反应以及感染的组织嗜性仅局限于被感染的黄地老虎宿主幼虫的脂肪体。对AgseGV - DA的基因组进行了全序列测定，并与中国分离株AgseGV - XJ和AgseGV - L1的全基因组序列进行了比较。所有这三个分离株都具有高度保守的基因组。AgseGV - DA基因组长度为131,557bp，编码149个推定的开放阅读框，包括37个杆状病毒核心基因和经口感染性因子ac110。对重复区域的全面研究确定了AgseGV - DA中一个推定的非同源重复序列（hr）样复制起点。基于37个杆状病毒核心基因的串联氨基酸比对以及基于部分颗粒体蛋白、lef - 8和lef - 9序列与已存档的β杆状病毒的核苷酸比对的成对距离进行的系统发育分析证实，AgseGV - DA、AgseGV - XJ和AgseGV - L1是同一β杆状病毒物种的代表性分离株。AgseGV编码一种独特的推定增强蛋白，与其他颗粒体病毒的增强蛋白亲缘关系较远。
