Fortes Maira Salomão, Lopes-Mori Fabiana Maria Ruiz, Caldart Eloiza Teles, Constantino Caroline, Evers Fernanda, Pagliari Sthefany, de Almeida Jonatas Campos, Barros Luiz Daniel, Freire Roberta Lemos, Garcia João Luis, Headley Selwyn Arlington, Navarro Italmar Teodorico
Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 380, Campus Universitário, PO Box 10.011, Paraná, 86057-970, Brazil.
School of Veterinary Medicine, Centro Universitário Filadélfia, Londrina, Paraná, Brazil.
Trop Anim Health Prod. 2018 Feb;50(2):413-419. doi: 10.1007/s11250-017-1450-1. Epub 2017 Oct 27.
This study investigated the prevalence of caprine toxoplasmosis in goat herds from Southern Brazil by the indirect immunofluorescence assay (IFA), and compared these results with the enzyme-linked immunosorbent assay (ELISA) and the modified agglutination test (MAT). In addition, possible risk factors associated with infection due to Toxoplasma gondii were determined. The serum samples evaluated were from 1,058 goats derived from 94 goat herds within the state of Paraná, Southern Brazil. Seropositivity by IFA was 30.0%, 33.3% by ELISA, and 25.3% by MAT. The risk factors associated with infection by T. gondii in goats were pasture rental, female goats, the presence of cats, and pastures shared with several goat herds. Using IFA as a standard, ELISA and MAT showed substantial concordance (kappa = 0.74 and 0.61), with sensitivities of 87 and 66.2% and specificities of 89.7 and 92.2%, respectively. These results demonstrate that caprine toxoplasmosis is endemic within the state of Paraná, Southern Brazil. In addition, the results from the three assays were relevant, without any significant differences as demonstrated due to the substantial concordance based on the kappa index.
本研究通过间接免疫荧光试验（IFA）调查了巴西南部山羊群中羊弓形虫病的流行情况，并将这些结果与酶联免疫吸附试验（ELISA）和改良凝集试验（MAT）进行了比较。此外，还确定了与弓形虫感染相关的可能风险因素。所评估的血清样本来自巴西南部巴拉那州94个山羊群的1058只山羊。IFA检测的血清阳性率为30.0%，ELISA为33.3%，MAT为25.3%。山羊感染弓形虫的风险因素包括租用牧场、母羊、有猫以及与多个山羊群共享的牧场。以IFA为标准，ELISA和MAT显示出高度一致性（kappa分别为0.74和0.61），敏感性分别为87%和66.2%，特异性分别为89.7%和92.2%。这些结果表明，羊弓形虫病在巴西南部的巴拉那州呈地方性流行。此外，三种检测方法的结果具有相关性，基于kappa指数的高度一致性表明没有任何显著差异。
