巴西圣保罗市蝙蝠弓形虫感染的血清流行病学研究

Seroepidemiology of Toxoplasma gondii infection in bats from São Paulo city, Brazil.

作者信息

Cabral Aline D, D'Auria Sandra R N, Camargo Maria C G O, Rosa Adriana R, Sodré Miriam M, Galvão-Dias Maria A, Jordão Luciana R, Dubey J P, Gennari Solange M, Pena Hilda F J

出版信息

Vet Parasitol. 2014 Dec 15;206(3-4):293-6. doi: 10.1016/j.vetpar.2014.10.014.

DOI:10.1016/j.vetpar.2014.10.014

PMID:25468027

Abstract

Toxoplasmosis is a parasitic zoonosis caused by Toxoplasma gondii protozoan with a worldwide distribution. The aim of this study was to determine the frequency of IgG anti-T. gondii antibodies in bats from São Paulo city, Brazil. A total of 616 serum samples were collected from 22 species of bats. Anti-T. gondii antibodies were searched using the indirect fluorescent antibody test (IFAT ≥ 1:16) and IgG anti-bat antibodies produced in sheep on samples collected during 2006-2011; 32.62% (201/616) of bats had T. gondii antibodies. The modified agglutination test (MAT ≥ 1:25) was performed on samples collected during 2010-2011; 18.61% (35/188) were seropositive. The concordance between IFAT and MAT (serum samples from 2010 to 2011) by Kappa (95% CI) was 0.144, resulting in a low agreement between the techniques. The specificity and sensitivity of MAT and IFAT have not been evaluated for the diagnosis of toxoplasmosis in bats. Thus, it was verified that bats are exposed to T. gondii during their lifetime and they are also part of the toxoplasmosis epidemiology.

摘要

弓形虫病是一种由刚地弓形虫原虫引起的寄生虫人畜共患病，在全球范围内均有分布。本研究的目的是确定巴西圣保罗市蝙蝠中抗弓形虫IgG抗体的频率。共从22种蝙蝠中采集了616份血清样本。使用间接荧光抗体试验（IFAT≥1:16）以及2006 - 2011年期间采集样本中羊产生的抗蝙蝠IgG抗体来检测抗弓形虫抗体；32.62%（201/616）的蝙蝠含有弓形虫抗体。对2010 - 2011年期间采集的样本进行改良凝集试验（MAT≥1:25）；18.61%（35/188）呈血清阳性。IFAT与MAT（2010年至2011年的血清样本）之间的Kappa一致性（95%CI）为0.144，表明这两种技术之间的一致性较低。MAT和IFAT在蝙蝠弓形虫病诊断中的特异性和敏感性尚未评估。因此，证实蝙蝠在其生命周期中会接触到弓形虫，并且它们也是弓形虫病流行病学的一部分。