用于精胺和亚精胺分析的基于胺氧化酶的生物传感器的开发。

Development of Amine-Oxidase-Based Biosensors for Spermine and Spermidine Analysis.

作者信息

Tortolini Cristina, Favero Gabriele, Mazzei Franco

机构信息

Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro, 500185, Rome, Italy.

出版信息

Methods Mol Biol. 2018;1694:75-80. doi: 10.1007/978-1-4939-7398-9_7.

DOI:10.1007/978-1-4939-7398-9_7

PMID:29080157

Abstract

In this work a detailed description of the development of amine oxidase-based electrochemical biosensors for the selective determination of the biogenic amines is presented. The enzymes required for this operation are Polyamine Oxidase (PAO) and Spermine Oxidase (SMO) which are physically entrapped in poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ), a photo-cross-linkable gel, onto screen printed electrode (SPE) surface. The developed biosensors are deeply characterized in the analysis of biogenic amines by using flow injection amperometric (FIA) technique. The enzymatic electrodes are characterized by good sensitivity, long-term stability, and reproducibility. To test the feasibility of the developed biosensors in the analysis of real matrices, they are used for the analysis of blood samples. The results obtained are in good agreement with those obtained with the GC-MS reference method.

摘要

在这项工作中，详细描述了用于选择性测定生物胺的基于胺氧化酶的电化学生物传感器的开发。该操作所需的酶是多胺氧化酶（PAO）和精胺氧化酶（SMO），它们被物理包埋在带有苯乙烯基吡啶鎓基团的聚乙烯醇（PVA-SbQ）中，这是一种可光交联的凝胶，涂覆在丝网印刷电极（SPE）表面。通过流动注射安培法（FIA）技术对开发的生物传感器在生物胺分析中进行了深入表征。酶电极具有良好的灵敏度、长期稳定性和重现性。为了测试开发的生物传感器在实际样品分析中的可行性，将它们用于血液样本的分析。获得的结果与采用气相色谱 - 质谱参考方法获得的结果高度一致。