Antoniou Chrystalla, Savvides Andreas, Georgiadou Egli C, Fotopoulos Vasileios
Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, P.O. Box 50329, 3603, Lemesos, Cyprus.
Methods Mol Biol. 2018;1694:155-161. doi: 10.1007/978-1-4939-7398-9_16.
Reactive oxygen, nitrogen and sulfur species are key signalling molecules involved in multiple physiological processes that can be examined in qualitative and quantitative manners. Here, we describe simple spectrophotometric assays that allow the quantification of hydrogen peroxide, nitrite-derived nitric oxide and hydrogen sulphide from plant tissues.
活性氧、氮和硫物质是参与多种生理过程的关键信号分子,这些生理过程可以通过定性和定量的方式进行检测。在此,我们描述了简单的分光光度法测定方法,可用于定量分析植物组织中的过氧化氢、亚硝酸盐衍生的一氧化氮和硫化氢。